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PMID: 17307854 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cytosolic proteins contribute to surface plasminogen recruitment of Neisseria meningitidis.

Journal of bacteriology ·Vol. 189 ·No. 8 ·2007-04-00 ·Pages 3246-55

Knaust A, Weber MV, Hammerschmidt S, Bergmann S, Frosch M, Kurzai O

Abstract

Plasminogen recruitment is a common strategy of pathogenic bacteria and results in a broad-spectrum surface-associated protease activity. Neisseria meningitidis has previously been shown to bind plasminogen. In this study, we show by several complementary approaches that endolase, DnaK, and peroxiredoxin, which are usually intracellular proteins, can also be located in the outer membrane and act as plasminogen receptors. Internal binding motifs, rather than C-terminal lysine residues, are responsible for plasminogen binding of the N. meningitidis receptors. Recombinant receptor proteins inhibit plasminogen association with N. meningitidis in a concentration-dependent manner. Besides binding purified plasminogen, N. meningitidis can also acquire plasminogen from human serum. Activation of N. meningitidis-associated plasminogen by urokinase results in functional activity and allows the bacteria to degrade fibrinogen. Furthermore, plasmin bound to N. meningitidis is protected against inactivation by alpha(2)-antiplasmin.

MeSH Terms
Amino Acid Motifs/physiology Bacterial Proteins/metabolism Binding Sites/physiology Cell Membrane/metabolism Humans Neisseria meningitidis/metabolism,pathogenicity Peroxidases/metabolism Peroxiredoxins Phosphopyruvate Hydratase/metabolism Plasminogen/metabolism Protein Binding Receptors, Cell Surface/metabolism Receptors, Urokinase Plasminogen Activator Virulence
Chemicals
Bacterial Proteins PLAUR protein, human Receptors, Cell Surface Receptors, Urokinase Plasminogen Activator Plasminogen Peroxidases Peroxiredoxins Phosphopyruvate Hydratase
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Knaust Andreas
Institute of Hygiene and Environmental Medicine, University of Giessen, Friedrichsstrasse 16, 35392 Giessen, Germany. Andreas.Knaust@hygiene.med.uni-giessen.de
Weber Martin V R
Hammerschmidt Sven
Bergmann Simone
Frosch Matthias
Kurzai Oliver
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.01966-06
Email
andreas.knaust@hygiene.med.uni-giessen.de
Published
2007-04-00
Epub
2007-00-16
Pages
3246-55
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC1855851
Subset
IM
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1128/JB.01966-06 PMC Full Text View journal details
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