Unique use of alternative polyadenylation signals in the mouse aldolase B gene.

Biochimica et biophysica acta ·Vol. 1129 ·No. 2 ·1992-01-06 ·Pages 243-5

Maine AB, Stauffer JK, Tolan DR, Ciejek-Baez E

Abstract

We isolated and sequenced two mouse aldolase B cDNAs. They differ only in the length of the 3' untranslated region. This is consistent with Northern blot analysis of liver RNA which shows two transcripts differing by 400 nucleotides. We also isolated and sequenced the corresponding 3' genomic region and found four polyadenylation signals in the final exon. RNase protection studies demonstrate that all four of these signals are utilized, but not equally. This is unique to the mouse aldolase B gene.

MeSH Terms

Animals Base Sequence Cloning, Molecular DNA Fructose-Bisphosphate Aldolase/genetics Genomic Library Isoenzymes/genetics Kidney/chemistry Liver/chemistry Mice Molecular Sequence Data Poly A/genetics RNA, Messenger/isolation & purification

Chemicals

Isoenzymes RNA, Messenger Poly A DNA Fructose-Bisphosphate Aldolase

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Maine A B

Department of Biochemistry, University of Rochester School of Medicine and Dentistry, NY 14642.

Stauffer J K

Tolan D R

Ciejek-Baez E

Article Info

Journal

Biochimica et biophysica acta

Abbr.

Biochim Biophys Acta

ISSN

0006-3002

DOI

10.1016/0167-4781(92)90497-n

Published

1992-01-06

Pages

243-5

Language

English

Region

Netherlands

NLM ID

0217513

Subset

Databases

GENBANK

D11333, D11334, M91424, M91425, M91426, M91427, M91428, M91429, M91430, X53402

External Links

PubMed source DOI: 10.1016/0167-4781(92)90497-n

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Unique use of alternative polyadenylation signals in the mouse aldolase B gene.

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