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PMID: 17270733 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Mechanisms of odor receptor gene choice in Drosophila.

Neuron ·Vol. 53 ·No. 3 ·2007-02-01 ·Pages 353-69

Ray A, van Naters Wv, Shiraiwa T, Carlson JR

Abstract

A remarkable problem in neurobiology is how olfactory receptor neurons (ORNs) select, from among a large odor receptor repertoire, which receptors to express. We use computational algorithms and mutational analysis to define positive and negative regulatory elements that are required for selection of odor receptor (Or) genes in the proper olfactory organ of Drosophila, and we identify an element that is essential for selection in one ORN class. Two odor receptors are coexpressed by virtue of the alternative splicing of a single gene, and we identify dicistronic mRNAs that each encode two receptors. Systematic analysis reveals no evidence for negative feedback regulation, but provides evidence that the choices made by neighboring ORNs of a sensillum are coordinated via the asymmetric segregation of regulatory factors from a common progenitor. We show that receptor gene choice in Drosophila also depends on a combinatorial code of transcription factors to generate the receptor-to-neuron map.

MeSH Terms
Alternative Splicing/genetics Animals Computational Biology DNA, Complementary/genetics Drosophila/genetics Electrophysiology Feedback/physiology Gene Expression/physiology Neurons/physiology Odorants Olfactory Receptor Neurons/physiology Regulatory Elements, Transcriptional Reverse Transcriptase Polymerase Chain Reaction Sense Organs/physiology Transcription Factors
Chemicals
DNA, Complementary Transcription Factors
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ray Anandasankar
Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520, USA.
van Naters Wynand van der Goes
Shiraiwa Takashi
Carlson John R
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Article Info
Journal
Neuron
Abbr.
Neuron
ISSN
0896-6273
DOI
10.1016/j.neuron.2006.12.010
Published
2007-02-01
Pages
353-69
Language
English
Region
United States
NLM ID
8809320
PMCID
PMC1986798
Subset
IM
Grants
NIDCD NIH HHS · R01 DC004729-04 · United States
NIDCD NIH HHS · R01 DC002174-19 · United States
NIDCD NIH HHS · R01 DC002174-18A1 · United States
NIDCD NIH HHS · R01 DC004729-05 · United States
NIDCD NIH HHS · R01 DC002174-22 · United States
NIDCD NIH HHS · DC04729 · United States
NIDCD NIH HHS · R01 DC004729-07 · United States
NIDCD NIH HHS · R01 DC002174-21 · United States
NIDCD NIH HHS · R01 DC002174-20 · United States
NIDCD NIH HHS · R01 DC002174 · United States
NIDCD NIH HHS · R01 DC004729 · United States
NIDCD NIH HHS · R01 DC004729-03 · United States
NIDCD NIH HHS · DC02174 · United States
NIDCD NIH HHS · R01 DC004729-06 · United States
External Links
PubMed source DOI: 10.1016/j.neuron.2006.12.010 PMC Full Text View journal details
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