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PMID: 17259981 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Direct activation of the ion channel TRPA1 by Ca2+.

Nature neuroscience ·Vol. 10 ·No. 3 ·2007-03-00 ·Pages 277-9

Zurborg S, Yurgionas B, Jira JA, Caspani O, Heppenstall PA

Abstract

TRPA1 is an ion channel expressed by nociceptors and activated by irritant compounds such as mustard oil. The endogenous function of TRPA1 has remained unclear, a fact highlighted by ongoing debate over its potential role as a sensor of noxious cold. Here we show that intracellular Ca(2+) activates human TRPA1 via an EF-hand domain and that cold sensitivity occurs indirectly (and nonphysiologically) through increased [Ca(2+)](i) during cooling in heterologous systems.

MeSH Terms

Analgesics, Non-Narcotic/pharmacology Calcium/metabolism Calcium Channels/physiology Carbachol/pharmacology Cell Line Cold Temperature Diagnostic Imaging/methods Dose-Response Relationship, Drug EF Hand Motifs/physiology Humans Intracellular Fluid/metabolism Luminescent Proteins/metabolism Membrane Proteins/physiology Mutagenesis/physiology Nerve Tissue Proteins/physiology Patch-Clamp Techniques/methods TRPA1 Cation Channel Transfection/methods Transient Receptor Potential Channels/drug effects,physiology,radiation effects

Chemicals

Analgesics, Non-Narcotic Calcium Channels Luminescent Proteins Membrane Proteins Nerve Tissue Proteins TRPA1 Cation Channel TRPA1 protein, human Transient Receptor Potential Channels Carbachol Calcium

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Zurborg Sandra

Klinik für Anaesthesiologie und operative Intensivmedizin, Charité Universitätsmedizin Berlin, Campus Benjamin Franklin, Hindenburgdamm 30, D-12200 Berlin, Germany.

Yurgionas Brian

Jira Julia A

Caspani Ombretta

Heppenstall Paul A

Article Info

Journal

Nature neuroscience

Abbr.

Nat Neurosci

ISSN

1097-6256

DOI

10.1038/nn1843

Published

2007-03-00

Epub

2007-00-28

Pages

277-9

Language

English

Region

United States

NLM ID

9809671

Subset

External Links

PubMed source DOI: 10.1038/nn1843 View journal details

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