Home LiteratureArticle Details
PMID: 1724075 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Effects of tachykinins on myenteric neurones of the guinea-pig gastric corpus: involvement of NK-3 receptors.

Pflugers Archiv : European journal of physiology ·Vol. 419 ·No. 6 ·1991-12-00 ·Pages 566-71

Schemann M, Kayser H

Abstract

Responses of gastric myenteric neurones evoked by the mammalian tachykinins substance P (SP), neurokinin A (NKA) and neurokinin B (NKB) were investigated using conventional intracellular recording methods. Application of the tachykinins caused a long lasting depolarization of the membrane potential which was associated with increased spike discharge and augmented excitability of the cells. The responses slowly desensitized. Additionally, cross desensitization occurred between SP, NKA and NKB. Both the NK-1 receptor agonist [Sar9,MetO2(11)]SP and the NK-2 receptor agonist [beta-Ala8]NKA(4-10) had no effect on the electrical properties of the neurones. Only the NK-3 receptor agonist [MePhe7]NKB mimicked the excitatory response observed during SP, NKA and NKB applications. [MePhe7]NKB-induced desensitization abolished the response to SP, NKA and NKB. However, long lasting applications of [Sar9,MetO2(11)]SP or [beta-Ala8]NKA(4-10) had no effect on the SP, NKA or NKB responses. The excitatory effect of SP, NKA and NKB remained unchanged during application of the tachykinin analogues [D-Arg1,D-Trp7,9,Leu11]SP and [Tyr5,D-Trp6,8,9,Arg10]NKA(4-10). The results indicate that SP, NKA and NKB act as excitatory neuromodulators within the enteric nervous system of the stomach. The effects of SP, NKA and NKB appeared to be mediated by activation of NK-3 receptors.

MeSH Terms
Animals Electrophysiology Guinea Pigs In Vitro Techniques Myenteric Plexus/drug effects,physiology Neurokinin A/pharmacology Neurokinin B/pharmacology Neurons/drug effects,physiology Receptors, Neurotransmitter/drug effects,physiology Receptors, Tachykinin Stomach/drug effects,innervation Substance P/pharmacology Tachykinins/pharmacology
Chemicals
Receptors, Neurotransmitter Receptors, Tachykinin Tachykinins Substance P Neurokinin A Neurokinin B
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Schemann M
Institute of Zoophysiology, University of Hohenheim, Stuttgart, Federal Republic of Germany.
Kayser H
References (30)
30 references, click to expand
  1. Electrical behaviour of myenteric neurones in the gastric corpus of the guinea-pig.
    J Physiol. 1989 Oct;417:501-18 PMID: 2621607
  2. Immunocytochemical analysis of potential neurotransmitters present in the myenteric plexus and muscular layers of the corpus of the guinea pig stomach.
    Anat Rec. 1989 Jul;224(3):431-42 PMID: 2476950
  3. Intracellular recording from neurones of the guinea-pig gall-bladder.
    J Physiol. 1990 Oct;429:323-38 PMID: 1703571
  4. Tachykinins: local gastric effects and brain stem responses.
    Am J Physiol. 1987 Mar;252(3 Pt 1):G365-73 PMID: 2435167
  5. Tachykinin receptors in the circular muscle of the guinea-pig ileum.
    Br J Pharmacol. 1990 Dec;101(4):996-1000 PMID: 1707710
  6. Effects of three mammalian tachykinins on single enteric neurons.
    Neurosci Lett. 1987 Nov 23;82(2):167-71 PMID: 2447535
  7. Inhibition of antidromically induced stimulation of gastric motility by substance P receptor blockade.
    Acta Physiol Scand. 1983 Aug;118(4):309-16 PMID: 6195890
  8. Observations on the actions of substance P and [D-Arg1,D-Pro2,D-Trp7,9,Leu11)substance P on single neurons of the guinea pig submucous plexus.
    Neuroscience. 1987 Jan;20(1):189-99 PMID: 2436087
  9. New selective agonists for neurokinin receptors: pharmacological tools for receptor characterization.
    Trends Pharmacol Sci. 1988 Aug;9(8):290-5 PMID: 2854928
  10. Contractile effects of substance P and neurokinin A on the rat stomach in vivo and in vitro.
    Br J Pharmacol. 1987 Jan;90(1):273-9 PMID: 2434177
  11. Actions of serotonin and substance P on myenteric neurons of guinea-pig distal colon.
    Eur J Pharmacol. 1988 Mar 22;148(1):1-8 PMID: 2454828
  12. Hexamethonium-resistant, atropine-sensitive vagal excitation of the feline stomach-activation of an unknown fibre system.
    Acta Physiol Scand. 1981 Aug;112(4):493-4 PMID: 7315429
  13. Ascending enteric reflex: multiple neurotransmitter systems and interactions.
    Am J Physiol. 1989 Mar;256(3 Pt 1):G540-5 PMID: 2466410
  14. Gastrointestinal tachykinin receptors.
    Life Sci. 1988;43(26):2203-8 PMID: 2850429
  15. Neurokinin B is a preferred agonist for a neuronal substance P receptor and its action is antagonized by enkephalin.
    Proc Natl Acad Sci U S A. 1985 Nov;82(21):7444-8 PMID: 2414777
  16. The actions of receptor-selective substance P analogs on myenteric neurons: an electrophysiological investigation.
    Eur J Pharmacol. 1988 Aug 24;153(2-3):247-53 PMID: 2460360
  17. Tachykinins produce fast and slow depolarizations in sympathetic neurons of rat coeliac-superior mesenteric ganglia.
    Brain Res. 1989 Jun 19;490(1):162-5 PMID: 2474359
  18. The actions of neurokinins and substance P in canine pylorus, antrum and duodenum.
    Peptides. 1989 May-Jun;10(3):671-9 PMID: 2476789
  19. Search for a physiological role of substance P in gastrointestinal motility.
    Neuroscience. 1985 Sep;16(1):1-32 PMID: 2423912
  20. Human isolated small intestine: motor responses of the longitudinal muscle to field stimulation and exogenous neuropeptides.
    Naunyn Schmiedebergs Arch Pharmacol. 1989 Apr;339(4):415-23 PMID: 2472555
  21. Reflex changes in circular muscle activity elicited by stroking the mucosa: an electrophysiological analysis in the isolated guinea-pig ileum.
    J Auton Nerv Syst. 1988 Dec;25(2-3):205-18 PMID: 2466886
  22. A novel radioimmunoassay for neuromedin K. I. Absence of neuromedin K-like immunoreactivity in guinea pig ileum and urinary bladder. II. Heterogeneity of tachykinins in guinea pig tissues.
    Regul Pept. 1989 Sep-Oct;26(2):93-105 PMID: 2798956
  23. In vivo and in vitro actions of mammalian tachykinins.
    Naunyn Schmiedebergs Arch Pharmacol. 1985 Aug;330(2):130-5 PMID: 2413373
  24. A highly selective NK-2 tachykinin receptor antagonist containing D-tryptophan.
    Eur J Pharmacol. 1990 Jan 3;175(1):113-5 PMID: 2157594
  25. Sensory vagal innervation of the rat esophagus and cardia: a light and electron microscopic anterograde tracing study.
    J Auton Nerv Syst. 1987 Oct;20(3):243-55 PMID: 3693803
  26. Does substance P mediate slow synaptic excitation within the myenteric plexus?
    Nature. 1978 Jul 27;274(5669):387-8 PMID: 672964
  27. Pharmacological analysis of [3H]-senktide binding to NK3 tachykinin receptors in guinea-pig ileum longitudinal muscle-myenteric plexus and cerebral cortex membranes.
    Br J Pharmacol. 1990 Apr;99(4):767-73 PMID: 1694464
  28. Electrical and synaptic properties of myenteric plexus neurones in the terminal large intestine of the guinea-pig.
    J Physiol. 1989 Aug;415:275-98 PMID: 2484208
  29. Effects of the putative substance P antagonists (D-PRO2, D-PHE7, D-TRP9, and D-PRO2, D-TRP7, D-TRP9) on electrical activity of myenteric neurons.
    J Auton Nerv Syst. 1983 Jun;8(2):165-9 PMID: 6194201
  30. Tachykinins modulate multiple ionic conductances in voltage-clamped rat spinal dorsal horn neurons.
    J Neurophysiol. 1989 Apr;61(4):854-65 PMID: 2470866
Article Info
Journal
Pflugers Archiv : European journal of physiology
Abbr.
Pflugers Arch
ISSN
0031-6768
DOI
10.1007/BF00370296
Published
1991-12-00
Pages
566-71
Language
English
Region
Germany
NLM ID
0154720
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00370296 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2