The multidrug-resistance gene MDR1 is expressed in human glial tumors.

Acta neuropathologica ·Vol. 82 ·No. 6 ·1991-00-00 ·Pages 516-9

Becker I, Becker KF, Meyermann R, Höllt V

Abstract

The most consistantly reported alteration of multidrug-resistant carcinoma cells is the overexpression of a membrane glycoprotein, termed P-glycoprotein. In this study we examined whether the strong intrinsic chemotherapy resistance of glial tumors might be related to the expression of the MDR1 gene which codes for P-glycoprotein. Fourteen glial tumors were examined immunohistochemically using the monoclonal antibody C219. In addition, RNA samples of 11 of these tumors were analysed using a sensitive Northern blot assay. P-glycoprotein is expressed in all 14 glial tumors; the number of stained tumor cells, however, varied considerably ranging from 0.3% to 15%. There was no correlation between the number of MDR1-positive cells and the histological malignancy. Varying amounts of MDR1 mRNA were detectable in 7 from 11 examined tumors. The results of our study show that the MDR1 gene is expressed in human glial tumors and suggest that the multidrug transporter may contribute to the clinical non-responsiveness of these tumors to chemotherapy.

MeSH Terms

ATP Binding Cassette Transporter, Subfamily B, Member 1 Blotting, Northern Brain Neoplasms/genetics,metabolism Drug Resistance/genetics Gene Expression Glioma/genetics,metabolism Humans Immunohistochemistry/methods Membrane Glycoproteins/genetics,metabolism RNA, Messenger/analysis Staining and Labeling

Chemicals

ATP Binding Cassette Transporter, Subfamily B, Member 1 Membrane Glycoproteins RNA, Messenger

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Becker I

Institut für Hirnforschung Universität Tübingen, Federal Republic of Germany.

Becker K F

Meyermann R

Höllt V

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Article Info

Journal

Acta neuropathologica

Abbr.

Acta Neuropathol

ISSN

0001-6322

DOI

10.1007/BF00293387

Published

1991-00-00

Pages

516-9

Language

English

Region

Germany

NLM ID

0412041

Subset

External Links

PubMed source DOI: 10.1007/BF00293387 View journal details

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The multidrug-resistance gene MDR1 is expressed in human glial tumors.

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