Projections from serotonin- and substance P-like immunoreactive neurons in the midbrain periaqueductal gray onto the nucleus reticularis gigantocellularis pars alpha in the rat.

Serotonin- and substance P-like immunoreactive (5HT-LI and SP-LI) neurons in the midbrain periaqueductal gray (PAG) of the rat were observed to send their axons to the nucleus reticularis gigantocellularis pars alpha (Rgc alpha) by the retrograde horseradish peroxidase (HRP)-tracing method combined with the 5HT- or SP-immunohistochemical technique. These 5HT- or SP-LI PAG neurons were distributed mainly in the ventrolateral subdivision and ventral portion of the medial subdivision at the middle and caudal levels of the PAG, and additionally in the nucleus raphe dorsalis (DR).