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PMID: 17209803 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Review

New insight into the biochemical mechanisms regulating auxin transport in plants.

The Biochemical journal ·Vol. 401 ·No. 3 ·2007-02-01 ·Pages 613-22

Kerr ID, Bennett MJ

Abstract

The transport of the plant hormone auxin has been under intense investigation since its identification 80 years ago. Studies have gradually refined our understanding of the importance of auxin transport in many aspects of plant signalling and development, and the focus has intensified in recent years towards the identification of the proteins involved in auxin transport and their functional mechanism. Within the past 18 months, the field has progressed rapidly, with confirmation that several distinct classes of proteins, previously dubbed as 'putative auxin permeases' or 'auxin transport facilitators', are bona fide transporters of IAA (indol-3-ylacetic acid). In this review we will appraise the recent transport data and highlight likely future research directions, including the characterization of auxiliary proteins necessary for the regulation of auxin transporters.

MeSH Terms
Biological Transport, Active Indoleacetic Acids/metabolism Plant Proteins/metabolism Plants/metabolism
Chemicals
Indoleacetic Acids Plant Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kerr Ian D
School of Biomedical Sciences, University of Nottingham, Queen's Medical Centre, Nottingham NG7 2UH, UK. kerr@nottingham.ac.uk
Bennett Malcolm J
References (96)
96 references, click to expand
  1. Molecular analysis of the Arabidopsis pattern formation of gene GNOM: gene structure and intragenic complementation.
    Mol Gen Genet. 1996 Apr 10;250(6):681-91 PMID: 8628228
  2. The translocation mechanism of P-glycoprotein.
    FEBS Lett. 2006 Feb 13;580(4):1056-63 PMID: 16380120
  3. Plant tropisms: the ins and outs of auxin.
    Curr Biol. 1996 Dec 1;6(12):1589-91 PMID: 8994821
  4. Agr, an Agravitropic locus of Arabidopsis thaliana, encodes a novel membrane-protein family member.
    Plant Cell Physiol. 1998 Oct;39(10):1111-8 PMID: 9871369
  5. Superfolding of the partially unfolded core-glycosylated intermediate of human P-glycoprotein into the mature enzyme is promoted by substrate-induced transmembrane domain interactions.
    J Biol Chem. 1998 Jun 12;273(24):14671-4 PMID: 9614062
  6. Auxin inhibits endocytosis and promotes its own efflux from cells.
    Nature. 2005 Jun 30;435(7046):1251-6 PMID: 15988527
  7. Drug binding sites on P-glycoprotein are altered by ATP binding prior to nucleotide hydrolysis.
    Biochemistry. 2000 Oct 3;39(39):11901-6 PMID: 11009602
  8. The mechanism of ammonia transport based on the crystal structure of AmtB of Escherichia coli.
    Proc Natl Acad Sci U S A. 2004 Dec 7;101(49):17090-5 PMID: 15563598
  9. ATPase and multidrug transport activities of the overexpressed yeast ABC protein Yor1p.
    J Biol Chem. 1998 May 15;273(20):12612-22 PMID: 9575223
  10. The Arabidopsis pxa1 mutant is defective in an ATP-binding cassette transporter-like protein required for peroxisomal fatty acid beta-oxidation.
    Plant Physiol. 2001 Nov;127(3):1266-78 PMID: 11706205
  11. AtPIN2 defines a locus of Arabidopsis for root gravitropism control.
    EMBO J. 1998 Dec 1;17(23):6903-11 PMID: 9843496
  12. Sites and regulation of auxin biosynthesis in Arabidopsis roots.
    Plant Cell. 2005 Apr;17(4):1090-104 PMID: 15772288
  13. AtSNX1 defines an endosome for auxin-carrier trafficking in Arabidopsis.
    Nature. 2006 Sep 7;443(7107):106-9 PMID: 16936718
  14. Arabidopsis AUX1 gene: a permease-like regulator of root gravitropism.
    Science. 1996 Aug 16;273(5277):948-50 PMID: 8688077
  15. Sequence of a gene cluster from Klebsiella pneumoniae encoding malonate decarboxylase and expression of the enzyme in Escherichia coli.
    Eur J Biochem. 1997 Jun 1;246(2):530-8 PMID: 9208947
  16. Auxin transport inhibitors block PIN1 cycling and vesicle trafficking.
    Nature. 2001 Sep 27;413(6854):425-8 PMID: 11574889
  17. Both P-glycoprotein nucleotide-binding sites are catalytically active.
    J Biol Chem. 1995 Nov 10;270(45):26956-61 PMID: 7592942
  18. Specialized membrane-localized chaperones prevent aggregation of polytopic proteins in the ER.
    J Cell Biol. 2005 Jan 3;168(1):79-88 PMID: 15623581
  19. The Arabidopsis GNOM ARF-GEF mediates endosomal recycling, auxin transport, and auxin-dependent plant growth.
    Cell. 2003 Jan 24;112(2):219-30 PMID: 12553910
  20. A receptor for auxin.
    Plant Cell. 2005 Sep;17(9):2425-9 PMID: 16141189
  21. Structure-function analysis of the presumptive Arabidopsis auxin permease AUX1.
    Plant Cell. 2004 Nov;16(11):3069-83 PMID: 15486104
  22. Structure and mechanism of ABC transporters.
    Curr Opin Struct Biol. 2002 Dec;12(6):754-60 PMID: 12504680
  23. The amino acid/auxin:proton symport permease family.
    Biochim Biophys Acta. 1999 Jan 8;1415(2):306-22 PMID: 9889387
  24. AUX1 regulates root gravitropism in Arabidopsis by facilitating auxin uptake within root apical tissues.
    EMBO J. 1999 Apr 15;18(8):2066-73 PMID: 10205161
  25. The discovery of the chemical nature of the plant hormone auxin.
    Riv Biol. 2002 May-Aug;95(2):289-308 PMID: 12449686
  26. Auxin transport.
    Curr Opin Plant Biol. 2005 Oct;8(5):494-500 PMID: 16054428
  27. Modeling alpha-helical transmembrane domains: the calculation and use of substitution tables for lipid-facing residues.
    Protein Sci. 1993 Jan;2(1):55-70 PMID: 8443590
  28. Immunophilin-like TWISTED DWARF1 modulates auxin efflux activities of Arabidopsis P-glycoproteins.
    J Biol Chem. 2006 Oct 13;281(41):30603-12 PMID: 16887800
  29. A draft sequence of the rice genome (Oryza sativa L. ssp. japonica).
    Science. 2002 Apr 5;296(5565):92-100 PMID: 11935018
  30. A new ER trafficking signal regulates the subunit stoichiometry of plasma membrane K(ATP) channels.
    Neuron. 1999 Mar;22(3):537-48 PMID: 10197533
  31. The PIN auxin efflux facilitators: evolutionary and functional perspectives.
    Trends Plant Sci. 2005 Apr;10(4):170-7 PMID: 15817418
  32. Sites and homeostatic control of auxin biosynthesis in Arabidopsis during vegetative growth.
    Plant J. 2001 Nov;28(4):465-74 PMID: 11737783
  33. Novel auxin transport inhibitors phenocopy the auxin influx carrier mutation aux1.
    Plant J. 2001 Feb;25(4):399-406 PMID: 11260496
  34. The human ATP-binding cassette (ABC) transporter superfamily.
    Genome Res. 2001 Jul;11(7):1156-66 PMID: 11435397
  35. A PINOID-dependent binary switch in apical-basal PIN polar targeting directs auxin efflux.
    Science. 2004 Oct 29;306(5697):862-5 PMID: 15514156
  36. Small GTPases in vesicle trafficking.
    Curr Opin Plant Biol. 2004 Dec;7(6):694-700 PMID: 15491918
  37. PGP4, an ATP binding cassette P-glycoprotein, catalyzes auxin transport in Arabidopsis thaliana roots.
    Plant Cell. 2005 Nov;17(11):2922-39 PMID: 16243904
  38. The Arabidopsis F-box protein TIR1 is an auxin receptor.
    Nature. 2005 May 26;435(7041):446-51 PMID: 15917798
  39. The F-box protein TIR1 is an auxin receptor.
    Nature. 2005 May 26;435(7041):441-5 PMID: 15917797
  40. The plant hormone indoleacetic acid induces invasive growth in Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 2004 Mar 23;101(12):4153-7 PMID: 15010530
  41. Mutational analysis of conserved carboxylate residues in the nucleotide binding sites of P-glycoprotein.
    Biochemistry. 2000 Nov 21;39(46):14138-49 PMID: 11087362
  42. TWISTED DWARF1, a unique plasma membrane-anchored immunophilin-like protein, interacts with Arabidopsis multidrug resistance-like transporters AtPGP1 and AtPGP19.
    Mol Biol Cell. 2003 Oct;14(10):4238-49 PMID: 14517332
  43. Inhibitors of the carrier-mediated influx of auxin in suspension-cultured tobacco cells.
    Planta. 2000 Mar;210(4):580-8 PMID: 10787051
  44. The arrangement of the transmembrane helices in the secretin receptor family of G-protein-coupled receptors.
    FEBS Lett. 1997 Jun 16;409(3):431-6 PMID: 9224704
  45. PINOID positively regulates auxin efflux in Arabidopsis root hair cells and tobacco cells.
    Plant Cell. 2006 Jul;18(7):1604-16 PMID: 16731587
  46. A draft sequence of the rice genome (Oryza sativa L. ssp. indica).
    Science. 2002 Apr 5;296(5565):79-92 PMID: 11935017
  47. Polar auxin transport--old questions and new concepts?
    Plant Mol Biol. 2002 Jun-Jul;49(3-4):273-84 PMID: 12036254
  48. Auxin transport: a field in flux.
    Trends Plant Sci. 2006 Aug;11(8):382-6 PMID: 16839804
  49. P-glycoprotein is stably inhibited by vanadate-induced trapping of nucleotide at a single catalytic site.
    J Biol Chem. 1995 Aug 18;270(33):19383-90 PMID: 7642618
  50. KATP channels as molecular sensors of cellular metabolism.
    Nature. 2006 Mar 23;440(7083):470-6 PMID: 16554807
  51. AXR4 is required for localization of the auxin influx facilitator AUX1.
    Science. 2006 May 26;312(5777):1218-20 PMID: 16690816
  52. Auxin transport: the fountain of life in plants?
    Dev Cell. 2003 Dec;5(6):824-6 PMID: 14667404
  53. Do phytotropins inhibit auxin efflux by impairing vesicle traffic?
    Plant Physiol. 2003 Jan;131(1):254-63 PMID: 12529533
  54. MDR-like ABC transporter AtPGP4 is involved in auxin-mediated lateral root and root hair development.
    FEBS Lett. 2005 Oct 10;579(24):5399-5406 PMID: 16198350
  55. Multiple drugbinding sites on the R482G isoform of the ABCG2 transporter.
    Br J Pharmacol. 2006 Nov;149(5):506-15 PMID: 16981002
  56. Evaluation of methods for the prediction of membrane spanning regions.
    Bioinformatics. 2001 Jul;17(7):646-53 PMID: 11448883
  57. Local, efflux-dependent auxin gradients as a common module for plant organ formation.
    Cell. 2003 Nov 26;115(5):591-602 PMID: 14651850
  58. Structure of a bacterial multidrug ABC transporter.
    Nature. 2006 Sep 14;443(7108):180-5 PMID: 16943773
  59. The ABC protein turned chloride channel whose failure causes cystic fibrosis.
    Nature. 2006 Mar 23;440(7083):477-83 PMID: 16554808
  60. Two homologous ATP-binding cassette transporter proteins, AtMDR1 and AtPGP1, regulate Arabidopsis photomorphogenesis and root development by mediating polar auxin transport.
    Plant Physiol. 2005 Jun;138(2):949-64 PMID: 15908594
  61. Auxin and cell polarity: the emergence of AXR4.
    Trends Plant Sci. 2006 Nov;11(11):517-8 PMID: 17030147
  62. The arabidopsis thaliana AGRAVITROPIC 1 gene encodes a component of the polar-auxin-transport efflux carrier.
    Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):15112-7 PMID: 9844024
  63. The PIN and LAX families of auxin transport genes in Medicago truncatula.
    Mol Genet Genomics. 2004 Nov;272(4):420-32 PMID: 15375694
  64. The TIR1 protein of Arabidopsis functions in auxin response and is related to human SKP2 and yeast grr1p.
    Genes Dev. 1998 Jan 15;12(2):198-207 PMID: 9436980
  65. The Arabidopsis thaliana ABC transporter AtMRP5 controls root development and stomata movement.
    EMBO J. 2001 Apr 17;20(8):1875-87 PMID: 11296221
  66. The Arabidopsis thaliana ABC protein superfamily, a complete inventory.
    J Biol Chem. 2001 Aug 10;276(32):30231-44 PMID: 11346655
  67. 3-D structural and functional characterization of the purified KATP channel complex Kir6.2-SUR1.
    EMBO J. 2005 Dec 7;24(23):4166-75 PMID: 16308567
  68. Regulators and effectors of the ARF GTPases.
    Curr Opin Cell Biol. 2000 Aug;12(4):475-82 PMID: 10873831
  69. PIN proteins perform a rate-limiting function in cellular auxin efflux.
    Science. 2006 May 12;312(5775):914-8 PMID: 16601150
  70. Cellular efflux of auxin catalyzed by the Arabidopsis MDR/PGP transporter AtPGP1.
    Plant J. 2005 Oct;44(2):179-94 PMID: 16212599
  71. Complete inventory of the yeast ABC proteins.
    Nat Genet. 1997 Feb;15(2):137-45 PMID: 9020838
  72. Structure and association of ATP-binding cassette transporter nucleotide-binding domains.
    Biochim Biophys Acta. 2002 Mar 19;1561(1):47-64 PMID: 11988180
  73. Involvement of an ABC transporter in a developmental pathway regulating hypocotyl cell elongation in the light
    Plant Cell. 1998 Oct;10(10):1623-36 PMID: 9761790
  74. Loss of an MDR transporter in compact stalks of maize br2 and sorghum dw3 mutants.
    Science. 2003 Oct 3;302(5642):81-4 PMID: 14526073
  75. Topology of NAT2, a prototypical example of a new family of amino acid transporters.
    J Biol Chem. 1997 Nov 28;272(48):30552-7 PMID: 9374550
  76. Carrier-mediated auxin transport.
    Planta. 1974 Jun;118(2):101-21 PMID: 24442257
  77. Analysis of the genome sequence of the flowering plant Arabidopsis thaliana.
    Nature. 2000 Dec 14;408(6814):796-815 PMID: 11130711
  78. The plant multidrug resistance ABC transporter AtMRP5 is involved in guard cell hormonal signalling and water use.
    Plant J. 2003 Jan;33(1):119-29 PMID: 12943546
  79. Membrane topology of human ABC proteins.
    FEBS Lett. 2006 Feb 13;580(4):1017-22 PMID: 16337630
  80. High-affinity auxin transport by the AUX1 influx carrier protein.
    Curr Biol. 2006 Jun 6;16(11):1123-7 PMID: 16677815
  81. Auxins.
    Vitam Horm. 2005;72:203-33 PMID: 16492472
  82. Root gravitropism requires lateral root cap and epidermal cells for transport and response to a mobile auxin signal.
    Nat Cell Biol. 2005 Nov;7(11):1057-65 PMID: 16244669
  83. Regulation of polar auxin transport by AtPIN1 in Arabidopsis vascular tissue.
    Science. 1998 Dec 18;282(5397):2226-30 PMID: 9856939
  84. An atomic detail model for the human ATP binding cassette transporter P-glycoprotein derived from disulfide cross-linking and homology modeling.
    FASEB J. 2003 Dec;17(15):2287-9 PMID: 14563687
  85. A gain-of-function mutation in the Arabidopsis pleiotropic drug resistance transporter PDR9 confers resistance to auxinic herbicides.
    Plant Physiol. 2006 Sep;142(1):63-74 PMID: 16877699
  86. Localization of the auxin permease AUX1 suggests two functionally distinct hormone transport pathways operate in the Arabidopsis root apex.
    Genes Dev. 2001 Oct 15;15(20):2648-53 PMID: 11641271
  87. Positively cooperative sites for drug transport by P-glycoprotein with distinct drug specificities.
    Eur J Biochem. 1997 Nov 15;250(1):130-7 PMID: 9432000
  88. The transporter associated with antigen processing TAP: structure and function.
    FEBS Lett. 1999 Dec 31;464(3):108-12 PMID: 10618487
  89. EIR1, a root-specific protein involved in auxin transport, is required for gravitropism in Arabidopsis thaliana.
    Genes Dev. 1998 Jul 15;12(14):2175-87 PMID: 9679062
  90. Involvement of CjMDR1, a plant multidrug-resistance-type ATP-binding cassette protein, in alkaloid transport in Coptis japonica.
    Proc Natl Acad Sci U S A. 2003 Jan 21;100(2):751-6 PMID: 12524452
  91. PIN and AUX/LAX proteins: their role in auxin accumulation.
    Trends Plant Sci. 2004 Dec;9(12):578-82 PMID: 15564124
  92. Enhanced gravi- and phototropism in plant mdr mutants mislocalizing the auxin efflux protein PIN1.
    Nature. 2003 Jun 26;423(6943):999-1002 PMID: 12827205
  93. EMB30 is essential for normal cell division, cell expansion, and cell adhesion in Arabidopsis and encodes a protein that has similarity to Sec7.
    Cell. 1994 Jul 1;77(7):1051-62 PMID: 8020095
  94. Multidrug resistance-like genes of Arabidopsis required for auxin transport and auxin-mediated development.
    Plant Cell. 2001 Nov;13(11):2441-54 PMID: 11701880
  95. Communication between multiple drug binding sites on P-glycoprotein.
    Mol Pharmacol. 2000 Sep;58(3):624-32 PMID: 10953057
  96. Cloning and characterization of the genes encoding the malolactic enzyme and the malate permease of Leuconostoc oenos.
    Appl Environ Microbiol. 1996 Apr;62(4):1274-82 PMID: 8919788
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
1470-8728
Published
2007-02-01
Pages
613-22
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1770846
Subset
IM
Grants
Biotechnology and Biological Sciences Research Council · BB/C514958/1 · United Kingdom
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