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PMID: 1720551 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Characterization of the two size forms of the alpha 1 subunit of skeletal muscle L-type calcium channels.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 23 ·1991-12-01 ·Pages 10778-82

De Jongh KS, Warner C, Colvin AA, Catterall WA

Abstract

The molecular properties of two size forms of the alpha 1 subunit of purified skeletal muscle calcium channels were analyzed. The minor, full-length, form, alpha 1(212), was found to have an apparent molecular mass of 214 kDa by Ferguson plot analysis, while the major, truncated, form, now designated alpha 1(190), had an apparent molecular mass of 193 kDa. Antibody mapping of the C-terminal region of alpha 1(190) with 10 anti-peptide antibodies placed the C terminus between residues 1685 and 1699. Three consensus sites for cAMP-dependent protein phosphorylation are present in the C-terminal region of alpha 1(212) but not in alpha 1(190), and they may be important for the regulation of the ion conductance activity of the calcium channel.

MeSH Terms
Amino Acid Sequence Animals Antibodies Calcium Channels/chemistry,metabolism Electrophoresis, Polyacrylamide Gel Epitopes/analysis Immunoblotting Macromolecular Substances Membrane Proteins/isolation & purification Microsomes/physiology Molecular Sequence Data Molecular Weight Muscles/physiology Peptide Mapping Peptides/chemical synthesis,immunology,isolation & purification Phosphorylation Protein Kinases
Chemicals
Antibodies Calcium Channels Epitopes Macromolecular Substances Membrane Proteins Peptides Protein Kinases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
De Jongh K S
Department of Pharmacology, University of Washington, Seattle 98195.
Warner C
Colvin A A
Catterall W A
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.23.10778
Published
1991-12-01
Pages
10778-82
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC53014
Subset
IM
Grants
NINDS NIH HHS · R0-1 NS22625 · United States
External Links
PubMed source DOI: 10.1073/pnas.88.23.10778 PMC Full Text View journal details
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