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PMID: 1718942 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Molecular cloning of a Pseudomonas paucimobilis gene encoding a 17-kilodalton polypeptide that eliminates HCl molecules from gamma-hexachlorocyclohexane.

Journal of bacteriology ·Vol. 173 ·No. 21 ·1991-11-00 ·Pages 6811-9

Imai R, Nagata Y, Fukuda M, Takagi M, Yano K

Abstract

Pseudomonas paucimobilis UT26 is capable of growing on gamma-hexachlorocyclohexane (gamma-HCH). A genomic library of P. paucimobilis UT26 was constructed in Pseudomonas putida by using the broad-host-range cosmid vector pKS13. After 2,300 clones were screened by gas chromatography, 3 clones showing gamma-HCH degradation were detected. A 5-kb fragment from one of the cosmid clones was subcloned into pUC118, and subsequent deletion and gas chromatography-mass spectrometry analyses revealed that a fragment of ca. 500 bp was responsible for the conversion of gamma-HCH to 1,2,4-trichlorobenzene via gamma-pentachlorocyclohexene. Nucleotide sequence analysis revealed an open reading frame (linA) of 465 bp within the fragment. The nucleotide sequence of the linA gene and the deduced amino acid sequence showed no similarity to any known sequences. The product of the linA gene was 16.5 kDa according to sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

Related Genes
linA
MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics,metabolism Base Sequence Blotting, Southern Cloning, Molecular Codon DNA, Bacterial Genes, Bacterial Hexachlorocyclohexane/analogs & derivatives,metabolism Hydrochloric Acid/metabolism Lyases/genetics,metabolism Mass Spectrometry Molecular Sequence Data Pseudomonas/enzymology,genetics,growth & development Restriction Mapping
Chemicals
Bacterial Proteins Codon DNA, Bacterial pentachlorocyclohexene Hexachlorocyclohexane Lyases dehydrochlorinases linA protein, Pseudomonas paucimobilis Hydrochloric Acid
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Imai R
Department of Agricultural Chemistry, University of Tokyo, Japan.
Nagata Y
Fukuda M
Takagi M
Yano K
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.173.21.6811-6819.1991
Published
1991-11-00
Pages
6811-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC209032
Subset
IM
Databases
GENBANK
D90355, M63251, M63252, M63253, M63254, M63255, S59272, S59273, S59274, S60892
External Links
PubMed source DOI: 10.1128/jb.173.21.6811-6819.1991 PMC Full Text View journal details
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