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PMID: 171661 Published · ppublish English Journal Article

Reversible effects of chaotropic agents on the proton permeability of Escherichia coli membrane vesicles.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 9 ·1975-09-00 ·Pages 3387-91

Patel L, Schuldiner S, Kaback HR

Abstract

Extraction of E. coli ML 308-225 membrane vesicles with chaotropic agents causes the vesicles to become specifically permeable to protons. As a result, the vesicles no longer generate a membrane potential, interior negative, and they do not catalyze respiration-dependent lactose or proline transport. Treatment of the extracted vesicles with various carbodiimides decreases the permeability of the vesicle membrane to protons, causing them to regain their ability to generate a membrane potential. By this means, active transport is completely reactivated. Exposure of the vesicles to carbodiimides prior to extraction with chaotropic agents makes transport activity impervious to the effects of the chaotropes.

MeSH Terms
Biological Transport, Active/drug effects Carbodiimides/pharmacology Cell Membrane Permeability/drug effects Cell-Free System Dose-Response Relationship, Drug Escherichia coli Guanidines/antagonists & inhibitors,pharmacology Membrane Potentials/drug effects Perchlorates/antagonists & inhibitors,pharmacology Protons Thiocyanates/antagonists & inhibitors,pharmacology Time Factors Urea/antagonists & inhibitors,pharmacology
Chemicals
Carbodiimides Guanidines Perchlorates Protons Thiocyanates Urea
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Patel L
Schuldiner S
Kaback H R
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38 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.9.3387
Published
1975-09-00
Pages
3387-91
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC432998
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.9.3387 PMC Full Text View journal details
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