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PMID: 17158748 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The histone variant mH2A1.1 interferes with transcription by down-regulating PARP-1 enzymatic activity.

Genes & development ·Vol. 20 ·No. 23 ·2006-12-01 ·Pages 3324-36

Ouararhni K, Hadj-Slimane R, Ait-Si-Ali S, Robin P, Mietton F, Harel-Bellan A, Dimitrov S, Hamiche A

Abstract

The histone variant mH2A is believed to be involved in transcriptional repression, but how it exerts its function remains elusive. By using chromatin immunoprecipitation and tandem affinity immunopurification of the mH2A1.1 nucleosome complex, we identified numerous genes with promoters containing mH2A1.1 nucleosomes. In particular, the promoters of the inducible Hsp70.1 and Hsp70.2 genes, but not that of the constitutively expressed Hsp70.8, were highly enriched in mH2A1.1. PARP-1 was identified as a part of the mH2A1.1 nucleosome complex and was found to be associated with the Hsp70.1 promoter. A specific interaction between mH2A1.1 and PARP-1 was demonstrated and found to be associated with inactivation of PARP-1 enzymatic activity. Heat shock released both mH2A1.1 and PARP-1 from the Hsp70.1 promoter and activated PARP-1 automodification activity. The data we present point to a novel mechanism for control of Hsp70.1 gene transcription. mH2A1.1 recruits PARP-1 to the promoter, thereby inactivating it. Upon heat shock, the Hsp70.1 promoter-bound PARP-1 is released to activate transcription through ADP-ribosylation of other Hsp70.1 promoter-bound proteins.

MeSH Terms
Chromatin/genetics,physiology Cloning, Molecular DNA Primers Gene Expression Regulation, Enzymologic Genetic Variation HSP70 Heat-Shock Proteins/genetics,metabolism HSP72 Heat-Shock Proteins/genetics,metabolism HeLa Cells Histones/genetics,isolation & purification,metabolism Humans Nucleosomes/physiology Poly (ADP-Ribose) Polymerase-1 Poly(ADP-ribose) Polymerases/genetics,metabolism Polymerase Chain Reaction RNA, Small Interfering/genetics Transcription, Genetic
Chemicals
Chromatin DNA Primers HSP70 Heat-Shock Proteins HSP72 Heat-Shock Proteins HSPA2 protein, human Histones Nucleosomes RNA, Small Interfering PARP1 protein, human Poly (ADP-Ribose) Polymerase-1 Poly(ADP-ribose) Polymerases
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Ouararhni Khalid
Laboratoire Epigénétique et Cancer, Centre National de la Recherche Scientifique FRE 2944, 94801 Villejuif, France.
Hadj-Slimane Réda
Ait-Si-Ali Slimane
Robin Philippe
Mietton Flore
Harel-Bellan Annick
Dimitrov Stefan
Hamiche Ali
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.396106
Published
2006-12-01
Pages
3324-36
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC1686608
Subset
IM
External Links
PubMed source DOI: 10.1101/gad.396106 PMC Full Text View journal details
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