Cloning and sequence analysis of a plasmid-encoded chloramphenicol acetyltransferase gene from Staphylococcus intermedius.

Journal of general microbiology ·Vol. 137 ·No. 4 ·1991-04-00 ·Pages 977-81

Schwarz S, Spies U, Cardoso M

Abstract

The chloramphenicol acetyltransferase gene (cat) of a 3.9 kb chloramphenicol resistance (CmR) plasmid from Staphylococcus intermedius, designated pSCS1, was cloned into an Escherichia coli plasmid vector. Sequence analysis revealed a high degree of base similarity with the cat gene of the S. aureus CmR plasmid pC221 but there were several differences in the regulatory region. A lesser degree of similarity was observed between the cat gene of the S. intermedius plasmid and the cat gene of the S. aureus plasmid pC194.

Related Genes

cat

MeSH Terms

Amino Acid Sequence Base Sequence Chloramphenicol O-Acetyltransferase/genetics,metabolism Cloning, Molecular DNA, Bacterial Genes, Bacterial Molecular Sequence Data Nucleic Acid Conformation Plasmids RNA, Bacterial RNA, Messenger Regulatory Sequences, Nucleic Acid Restriction Mapping Sequence Homology, Nucleic Acid Staphylococcus/enzymology,genetics

Chemicals

DNA, Bacterial RNA, Bacterial RNA, Messenger Chloramphenicol O-Acetyltransferase

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Schwarz S

Institut für Bakteriologie und Immunologie, Justus-Liebig-Universität Giessen, FRG.

Spies U

Cardoso M

Article Info

Journal

Journal of general microbiology

Abbr.

J Gen Microbiol

ISSN

0022-1287

DOI

10.1099/00221287-137-4-977

Published

1991-04-00

Pages

977-81

Language

English

Region

England

NLM ID

0375371

Subset

Databases

GENBANK

M38702, M59337, M59338, M59339, M59340, M59341, M59342, M59343, M59344, M63943, M64281

External Links

PubMed source DOI: 10.1099/00221287-137-4-977

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Cloning and sequence analysis of a plasmid-encoded chloramphenicol acetyltransferase gene from Staphylococcus intermedius.

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