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PMID: 1713197 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Molecular conservation of the P6 outer membrane protein among strains of Haemophilus influenzae: analysis of antigenic determinants, gene sequences, and restriction fragment length polymorphisms.

Infection and immunity ·Vol. 59 ·No. 8 ·1991-08-00 ·Pages 2658-63

Nelson MB, Munson RS, Apicella MA, Sikkema DJ, Molleston JP, Murphy TF

Abstract

Infections caused by Haemophilus influenzae are a major worldwide health problem. In particular, nontypeable strains of H. influenzae are a common cause of otitis media in infants and children. A vaccine to prevent these infections would result in the prevention of substantial morbidity and cost savings. A problem in identifying an appropriate vaccine antigen has been the enormous antigenic heterogeneity among nontypeable strains of H. influenzae. The present study was undertaken to characterize the conservation of the P6 outer membrane protein (approximately 16,000 daltons) among strains of H. influenzae. A total of 20 type b strains and 20 nontypeable strains of diverse geographic and clinical origins was studied. Three approaches were taken. (i) Antigenic determinants recognized by monoclonal and polyclonal antibodies were present on P6 in all 40 strains tested. The molecular weight of P6 was identical in all strains. (ii) Comparison of the DNA sequences of the P6 genes from three epidemiologically and serologically unrelated strains demonstrated 100% homology at the amino acid level and 97 to 99% homology at the nucleotide level. (iii) Restriction fragment length polymorphism analysis demonstrated that the P6 gene and flanking sequences were highly conserved among all strains. These three independent series of experiments indicated that the P6 protein is highly conserved among strains of H. influenzae. P6 should receive serious consideration for inclusion in a vaccine to prevent infections caused by nontypeable H. influenzae.

Related Genes
P6
MeSH Terms
Amino Acid Sequence Antibodies, Bacterial/immunology Antibodies, Monoclonal/immunology Antigens, Bacterial/genetics,immunology Bacterial Outer Membrane Proteins/genetics,immunology Base Sequence Blotting, Southern DNA, Bacterial Epitopes/immunology Genes, Bacterial Haemophilus Vaccines Haemophilus influenzae/genetics,immunology Immunoblotting Molecular Sequence Data Polymorphism, Restriction Fragment Length Restriction Mapping Sequence Homology, Nucleic Acid
Chemicals
Antibodies, Bacterial Antibodies, Monoclonal Antigens, Bacterial Bacterial Outer Membrane Proteins DNA, Bacterial Epitopes Haemophilus Vaccines P6 outer membrane protein, Haemophilus
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Nelson M B
Department of Medicine, State University of New York, Buffalo.
Munson R S
Apicella M A
Sikkema D J
Molleston J P
Murphy T F
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.59.8.2658-2663.1991
Published
1991-08-00
Pages
2658-63
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC258070
Subset
IM
Grants
NIAID NIH HHS · AI 17572 · United States
NIAID NIH HHS · AI 19641 · United States
External Links
PubMed source DOI: 10.1128/iai.59.8.2658-2663.1991 PMC Full Text View journal details
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