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PMID: 17123111 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Wheat VIN3-like PHD finger genes are up-regulated by vernalization.

Molecular genetics and genomics : MGG ·Vol. 277 ·No. 3 ·2007-03-00 ·Pages 301-13

Fu D, Dunbar M, Dubcovsky J

Abstract

The term 'vernalization' describes the acceleration of the transition between the vegetative and reproductive stages after exposing plants to an extended period of low temperature. In Arabidopsis, vernalization promotes flowering by silencing the flowering repressor gene FLOWERING LOCUS C (FLC). Mitotically stable repression of FLC is the result of chromatin modifications mediated by the Vernalization-INsensitive 3 (VIN3) and VIN3-Like (VIL) proteins. In this study, we identified and characterized three VIL genes in diploid wheat (Triticum monococcum L.), named TmVIL1, TmVIL2, and TmVIL3. Similar to Arabidopsis VIN3, all three wheat VIL proteins carry three conserved domains including a plant homeodomain finger motif (PHD), a fibronectin type III domain (FNIII), and a VIN3 interacting domain (VID). Genetic mapping placed TmVIL1, TmVIL2, and TmVIL3 loci in the centromeric regions of chromosome 5, 6, and 1, respectively. The chromosome location of TmVIL1 is close to that of the vernalization gene VRN-D5, but more precise mapping information is required to validate this relationship. Transcription of the wheat VIL genes was up-regulated by vernalization, with a peak after 4-6 weeks of cold treatment. When transferred back to warm conditions, transcript levels of the wheat VIL genes returned to pre-vernalization levels. In addition, the transcript levels of wheat VIL genes are affected by photoperiod. This study indicates that wheat VIL genes have retained a similar structure and transcriptional regulation as their Arabidopsis VIN3/VIL homologues, suggesting that they might have retained some of their functions.

MeSH Terms
Acclimatization/genetics Amino Acid Sequence Base Sequence Chromosome Mapping Chromosomes, Plant/genetics Cloning, Molecular DNA Primers/genetics DNA, Plant/genetics Gene Expression Profiling Gene Expression Regulation, Plant Genes, Plant Molecular Sequence Data Photoperiod Phylogeny Plant Proteins/genetics Sequence Homology, Amino Acid Triticum/genetics,physiology Up-Regulation
Chemicals
DNA Primers DNA, Plant Plant Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Fu Daolin
Department of Plant Sciences, University of California, One Shields Av., Davis, CA 95616, USA.
Dunbar Mignon
Dubcovsky Jorge
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Article Info
Journal
Molecular genetics and genomics : MGG
Abbr.
Mol Genet Genomics
ISSN
1617-4615
DOI
10.1007/s00438-006-0189-6
Published
2007-03-00
Epub
2006-00-23
Pages
301-13
Language
English
Region
Germany
NLM ID
101093320
Subset
IM
External Links
PubMed source DOI: 10.1007/s00438-006-0189-6 View journal details
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