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PMID: 17123104 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The analysis of the ChlI 1 and ChlI 2 genes using acifluorfen-resistant mutant of Arabidopsis thaliana.

Planta ·Vol. 225 ·No. 4 ·2007-03-00 ·Pages 935-43

Apchelimov AA, Soldatova OP, Ezhova TA, Grimm B, Shestakov SV

Abstract

One of the key regulatory enzymes of the chlorophyll biosynthesis pathway is magnesium (Mg) chelatase, consisting of three different subunits CHLI, CHLD and CHLH. While CHLH and CHLD are encoded by a single gene each in Arabidopsis, CHLI is encoded by two homologous genes, ChlI 1 and ChlI 2. Analysis of the acifluorfen herbicide resistant mutant aci5 revealed an alteration of the ChlI 1 gene. This mutant as well as wild type plants contained similar transcript levels of the ChlI 1 and ChlI 2 genes. Moreover, the transcripts of both alleles of the ChlI 1 gene were present in the cs (ch42-2)/aci5 hybrid which showed an albina phenotype. Comparison of the amino acid sequence of CHLI 1 and CHLI 2 encoded in the genome of aci5 and wild type revealed in particular alterations of the C-terminal end which are suggested to be responsible for the decreased ability of CHLI 2 to participate in the formation of the CHLI ring-like structure of the Mg chelatase complex.

MeSH Terms
Amino Acid Sequence Arabidopsis/genetics,ultrastructure Chloroplasts/ultrastructure Herbicide Resistance Herbicides Heterozygote Homozygote Lyases/genetics Microscopy, Electron, Transmission Molecular Sequence Data Mutation Nitrobenzoates Phenotype Protein Structure, Quaternary Sequence Analysis, Protein Sequence Homology, Amino Acid Transcription, Genetic
Chemicals
Herbicides Nitrobenzoates Lyases magnesium chelatase acifluorfen
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Apchelimov Alexey A
Biology Faculty/Department of Genetics, M.V. Lomonosov Moscow State University, Moscow, 119899, Russia. apchelimov@mail.ru
Soldatova Olga P
Ezhova Tatiana A
Grimm Bernhard
Shestakov Sergey V
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Article Info
Journal
Planta
Abbr.
Planta
ISSN
0032-0935
DOI
10.1007/s00425-006-0390-1
Email
apchelimov@mail.ru
Published
2007-03-00
Pages
935-43
Language
English
Region
Germany
NLM ID
1250576
Subset
IM
External Links
PubMed source DOI: 10.1007/s00425-006-0390-1 View journal details
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