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PMID: 17121391 Published · ppublish English Journal Article

A semiautomatic instrument for the radioautographic coating technique.

Kopriwa BM

Abstract

By means of a mechanical coating instrument a fast, simple method to coat specimens with liquid nuclear track emulsion has been devised for quantitative light and electron microscopic radioautography. In both cases, the section is mounted on a glass slide. After the vertically held slide has been immersed in the melted emulsion, the instrument withdraws it at a slow, constant speed. As a result, the specimen is coated with a thin, uniform emulsion layer composed of homogeneously distributed silver bromide crystals. The thickness of the emulsion coat may be standardized by selection of an optimal combination of emulsion dilution, temperature and withdrawal speed.

MeSH Terms
Autoradiography/instrumentation,methods Bromides/chemistry Emulsions/chemistry Gelatin/chemistry Liver/chemistry,ultrastructure Microscopy, Electron/instrumentation,methods Sensitivity and Specificity Silver Compounds/chemistry Specimen Handling/methods Surface Properties
Chemicals
Bromides Emulsions Silver Compounds Gelatin silver bromide
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Kopriwa B M
Department of Anatomy, McGill University, Montreal, Canada.
Article Info
Journal
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
Abbr.
J Histochem Cytochem
ISSN
0022-1554
Published
1966-12-00
Pages
923-8
Language
English
Region
United States
NLM ID
9815334
Subset
IM
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