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PMID: 1712105 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Identification of type-specific linear epitopes in the glycoproteins gp46 and gp21 of human T-cell leukemia viruses type I and type II using synthetic peptides.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 13 ·1991-07-01 ·Pages 5754-8

Horal P, Hall WW, Svennerholm B, Lycke J, Jeansson S, Rymo L, Kaplan MH, Vahlne A

Abstract

Synthetic peptides of 20-25 amino acids were employed in enzyme-linked immunosorbent assays to identify linear epitopes in the external glycoprotein gp46 and the transmembrane glycoprotein gp21 of human T-cell leukemia/lymphotropic viruses type I (HTLV-I) and II (HTLV-II). Ten linear epitopes were identified in the HTLV-I glycoproteins, seven in gp46 and three in gp21. Three major linear epitopes were identified in the gp46 of HTLV-II. Peptides representing linear epitopes of gp46 were found to be sensitive and specific for the detection of antibody and permit serological identification and differentiation of HTLV-I and HTLV-II infections.

MeSH Terms
Amino Acid Sequence Antibody Specificity Deltaretrovirus Antibodies/immunology Deltaretrovirus Antigens/immunology Epitopes Gene Products, env/immunology HTLV-I Infections/immunology HTLV-II Infections/immunology Human T-lymphotropic virus 1/immunology Human T-lymphotropic virus 2/immunology Humans Molecular Sequence Data Retroviridae Proteins, Oncogenic/immunology Species Specificity env Gene Products, Human Immunodeficiency Virus
Chemicals
Deltaretrovirus Antibodies Deltaretrovirus Antigens Epitopes Gene Products, env Retroviridae Proteins, Oncogenic env Gene Products, Human Immunodeficiency Virus gp21 protein, Human T-lymphotropic virus 1 gp46 protein, Human T-cell leukemia virus type I gp46 protein, Human immunodeficiency virus 2 human T-cell leukemia virus type-II protein gp21
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Horal P
Department of Clinical Virology, University of Göteborg, Sweden.
Hall W W
Svennerholm B
Lycke J
Jeansson S
Rymo L
Kaplan M H
Vahlne A
References (31)
31 references, click to expand
  1. Type-specific antigens for serological discrimination of HTLV-I and HTLV-II infection.
    Lancet. 1990 Nov 10;336(8724):1153-5 PMID: 1978028
  2. A new subtype of human T-cell leukemia virus (HTLV-II) associated with a T-cell variant of hairy cell leukemia.
    Science. 1982 Nov 5;218(4572):571-3 PMID: 6981847
  3. Prevalence of human T-cell leukemia/lymphoma virus (HTLV) type II infection among high-risk individuals: type-specific identification of HTLVs by polymerase chain reaction.
    Blood. 1989 Oct;74(5):1658-64 PMID: 2790192
  4. Seroprevalence and epidemiological correlates of HTLV-I infection in U.S. blood donors.
    Science. 1988 Apr 29;240(4852):643-6 PMID: 2896386
  5. High rate of HTLV-II infection in seropositive i.v. drug abusers in New Orleans.
    Science. 1989 Apr 28;244(4903):471-5 PMID: 2655084
  6. Antibodies to human T-lymphotropic virus type-I in patients with tropical spastic paraparesis.
    Lancet. 1985 Aug 24;2(8452):407-10 PMID: 2863442
  7. HTLV-I associated myelopathy, a new clinical entity.
    Lancet. 1986 May 3;1(8488):1031-2 PMID: 2871307
  8. Integrated human T-cell leukemia virus II genome in CD8 + T cells from a patient with "atypical" hairy cell leukemia: evidence for distinct T and B cell lymphoproliferative disorders.
    Blood. 1988 Feb;71(2):363-9 PMID: 2827811
  9. Discrimination between antibodies to HIV and to related retroviruses using site-directed serology.
    Nature. 1987 Sep 17-23;329(6136):248-50 PMID: 3041232
  10. A second isolate of HTLV-II associated with atypical hairy-cell leukemia.
    N Engl J Med. 1986 Aug 7;315(6):372-7 PMID: 3016537
  11. The family of human T-lymphotropic leukemia viruses: HTLV-I as the cause of adult T cell leukemia and HTLV-III as the cause of acquired immunodeficiency syndrome.
    Blood. 1985 Feb;65(2):253-63 PMID: 2981583
  12. The chemistry of site-directed serology for HIV infections.
    AIDS Res Hum Retroviruses. 1989 Oct;5(5):487-93 PMID: 2480152
  13. Synthetic env gp41 peptide as a sensitive and specific diagnostic reagent in different stages of human immunodeficiency virus type 1 infection.
    J Med Virol. 1988 Oct;26(2):111-8 PMID: 2460585
  14. Fine mapping of an immunodominant domain in the transmembrane glycoprotein of human immunodeficiency virus.
    J Virol. 1987 Aug;61(8):2639-41 PMID: 2439707
  15. Diagnosis of AIDS by using a 12-amino acid peptide representing an immunodominant epitope of the human immunodeficiency virus.
    J Infect Dis. 1987 Aug;156(2):261-7 PMID: 2439614
  16. Envelope proteins of human T cell leukemia virus type I: characterization by antisera to synthetic peptides and identification of a natural epitope.
    J Immunol. 1986 Nov 1;137(9):2945-51 PMID: 2428880
  17. C-terminal region of human T cell lymphotropic virus type I (HTLVI) p19 core protein is immunogenic in humans and contains an HTLVI-specific epitope.
    J Immunol. 1986 Apr 1;136(7):2393-7 PMID: 2419433
  18. Detection of antibodies to human T-lymphotropic virus type III by using a synthetic peptide of 21 amino acid residues corresponding to a highly antigenic segment of gp41 envelope protein.
    Proc Natl Acad Sci U S A. 1986 Aug;83(16):6159-63 PMID: 2426711
  19. Complete nucleotide sequence of an infectious clone of human T-cell leukemia virus type II: an open reading frame for the protease gene.
    Proc Natl Acad Sci U S A. 1985 May;82(10):3101-5 PMID: 2582407
  20. Mapping of immunogenic regions of human T cell leukemia virus type I (HTLV-I) gp46 and gp21 envelope glycoproteins with env-encoded synthetic peptides and a monoclonal antibody to gp46.
    J Immunol. 1989 Feb 1;142(3):971-8 PMID: 2563272
  21. Antibody reactivity to different regions of human T-cell leukemia virus type 1 gp61 in infected people.
    J Virol. 1989 Nov;63(11):4952-7 PMID: 2677406
  22. Infection with HTLV-I and HTLV-II: evolving concepts.
    Semin Hematol. 1988 Jul;25(3):230-46 PMID: 2900553
  23. Synthetic peptide immunoassay distinguishes HIV type 1 and HIV type 2 infections.
    Science. 1987 Sep 11;237(4820):1346-9 PMID: 2888192
  24. Site-directed ELISA with synthetic peptides representing the HIV transmembrane glycoprotein.
    J Med Virol. 1987 Sep;23(1):1-9 PMID: 3500271
  25. Antibody to a synthetic oligopeptide in subjects at risk for human immunodeficiency virus infection.
    J Clin Microbiol. 1987 Aug;25(8):1498-504 PMID: 3476491
  26. Determination of a unique and immunodominant epitope of human T cell lymphotropic virus type I.
    J Infect Dis. 1990 Aug;162(2):353-7 PMID: 1695653
  27. Low prevalence in the UK of HTLV-I and HTLV-II infection in subjects with AIDS, with extended lymphadenopathy, and at risk of AIDS.
    Lancet. 1984 Jul 21;2(8395):125-8 PMID: 6146034
  28. Detection and isolation of type C retrovirus particles from fresh and cultured lymphocytes of a patient with cutaneous T-cell lymphoma.
    Proc Natl Acad Sci U S A. 1980 Dec;77(12):7415-9 PMID: 6261256
  29. Human adult T-cell leukemia virus: complete nucleotide sequence of the provirus genome integrated in leukemia cell DNA.
    Proc Natl Acad Sci U S A. 1983 Jun;80(12):3618-22 PMID: 6304725
  30. Type C virus particles in a cord T-cell line derived by co-cultivating normal human cord leukocytes and human leukaemic T cells.
    Nature. 1981 Dec 24;294(5843):770-1 PMID: 6275274
  31. Comparison of Western immunoblot antigens and interpretive criteria for detection of antibody to human T-lymphotropic virus types I and II.
    J Clin Microbiol. 1990 Sep;28(9):2045-50 PMID: 1977764
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.13.5754
Published
1991-07-01
Pages
5754-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC51956
Subset
IM
Grants
NCI NIH HHS · CA51012-01A1 · United States
External Links
PubMed source DOI: 10.1073/pnas.88.13.5754 PMC Full Text View journal details
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