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PMID: 1711237 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Increased susceptibility to mouse hepatitis virus type 3 (MHV3) infection induced by a hypercholesterolaemic diet with increased adsorption of MHV3 to primary hepatocyte cultures.

Research in virology ·Vol. 142 ·No. 1 ·1991-00-00 ·Pages 5-15

Braunwald J, Nonnenmacher H, Pereira CA, Kirn A

Abstract

The administration of a hypercholesterolaemic (HC) diet rendered genetically resistant A/J mice susceptible to mouse hepatitis virus 3 (MHV3) infection. The animals died of acute hepatitis with high viral titres in the liver accompanied by many necrotic foci and high serum transaminase levels. Resistance to virus was re-established by refeeding HC mice with a normal diet for 2 weeks. This modification of pathogenesis was accompanied by an increase in the susceptibility of hepatocyte cultures from HC mice to MHV3 and could be explained by an enhancement in virus adsorption. We hypothesize that the incorporation of cholesterol into the plasma membranes of hepatocytes of HC mice, thereby decreasing the membrane fluidity, may lead to an increase in the availability of virus receptors.

MeSH Terms
Adsorption Animals Cells, Cultured Cholesterol, Dietary/pharmacology Disease Susceptibility Fluorescent Antibody Technique Hepatitis, Viral, Animal/immunology Interferons/pharmacology Liver/microbiology Mice Mice, Inbred A Murine hepatitis virus/pathogenicity RNA, Viral/biosynthesis Radioimmunoassay Viral Proteins/biosynthesis Virus Replication/drug effects
Chemicals
Cholesterol, Dietary RNA, Viral Viral Proteins Interferons
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Braunwald J
Laboratoire de Virologie de la Faculté de Médecine, Université Louis Pasteur et INSERM U74, Strasbourg, France.
Nonnenmacher H
Pereira C A
Kirn A
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Article Info
Journal
Research in virology
Abbr.
Res Virol
ISSN
0923-2516
DOI
10.1016/0923-2516(91)90022-u
Published
1991-00-00
Pages
5-15
Language
English
Region
France
NLM ID
8907469
PMCID
PMC7135346
Subset
IM
External Links
PubMed source DOI: 10.1016/0923-2516(91)90022-u PMC Full Text
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