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PMID: 17086442 Published · ppublish English Journal Article

Paraquat-induced oxidative stress in drosophila melanogaster: effects of melatonin, glutathione, serotonin, minocycline, lipoic acid and ascorbic acid.

Neurochemical research ·Vol. 31 ·No. 12 ·2006-12-00 ·Pages 1425-32

Bonilla E, Medina-Leendertz S, Villalobos V, Molero L, Bohórquez A

Abstract

The efficacy of melatonin, glutathione, serotonin, minocycline, lipoic acid and ascorbic acid in counteracting the toxicity of paraquat in Drosophila melanogaster was examined. Male Oregon wild strain flies were fed for 5 days with control food or food containing the test substance. They were transferred in groups of five to vials containing only filter paper soaked with 20 mM paraquat in 5% sucrose solution. Survival was determined 24 and 48 h later. All the substances assayed increased the survival of D. melanogaster. At equimolar concentrations (0.43 mM) melatonin was more effective than serotonin, lipoic acid and ascorbic acid. However, lower concentrations of glutathione (0.22 mM) and minocycline (0.05 mM) were as efficient as melatonin. The highest survival rate (38.6%) after 48 h of paraquat treatment was found with 2.15 mM of lipoic acid. No synergistic effect of melatonin with glutathione, serotonin, minocycline, lipoic acid and ascorbic acid was detected.

MeSH Terms
Animals Antioxidants/metabolism,pharmacology Ascorbic Acid/metabolism,pharmacology Drosophila melanogaster Drug Resistance Glutathione/metabolism,pharmacology Herbicides/antagonists & inhibitors,toxicity Male Melatonin/metabolism,pharmacology Minocycline/metabolism,pharmacology Oxidative Stress/drug effects Paraquat/antagonists & inhibitors,toxicity Serotonin/metabolism,pharmacology Survival Rate Thioctic Acid/metabolism,pharmacology
Chemicals
Antioxidants Herbicides Serotonin Thioctic Acid Minocycline Glutathione Melatonin Paraquat Ascorbic Acid
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Bonilla Ernesto
Neurochemistry Section, Instituto de Investigaciones Clínicas Dr Américo Negrette, Universidad del Zulia, Apartado 23, Maracaibo, Venezuela. ebonillaro@yahoo.com
Medina-Leendertz Shirley
Villalobos Virginia
Molero Leunardy
Bohórquez Aquiles
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Article Info
Journal
Neurochemical research
Abbr.
Neurochem Res
ISSN
0364-3190
DOI
10.1007/s11064-006-9194-8
Email
ebonillaro@yahoo.com
Published
2006-12-00
Epub
2006-00-04
Pages
1425-32
Language
English
Region
United States
NLM ID
7613461
Subset
IM
External Links
PubMed source DOI: 10.1007/s11064-006-9194-8 View journal details
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