Isolation of a second S-locus-related cDNA from Brassica oleracea: genetic relationships between the S locus and two related loci.
Self-incompatibility in Brassica oleracea is controlled by the highly polymorphic S locus. Isolation and subsequent characterization of the S-locus-glycoprotein (SLG) gene, which encodes the S-locus-specific glycoprotein (SLSG), has revealed the presence of a self-incompatibility multigene family. One of these S-locus-related genes, SLR1, has been shown to be expressed. In this study we present the isolation and preliminary characterization of a second expressed S-locus-related sequence, SLR2. Through restriction fragment length polymorphism (RFLP) linkage analysis we demonstrate that the SLR1 and SLR2 loci reside approximately 18.5 map units apart in one linkage group that segregates independently of the S-locus. The identification of a second SLR gene expressed in stigmas suggests that loci unlinked to the S-locus may play a role in the self-incompatibility response, or in pollination in general.
11 references, click to expand
-
DNA sequencing with chain-terminating inhibitors.Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
-
Detection of specific sequences among DNA fragments separated by gel electrophoresis.J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
-
A new class of S sequences defined by a pollen recessive self-incompatibility allele of Brassica oleracea.Mol Gen Genet. 1990 Jul;222(2-3):241-8 PMID: 1980334
-
A cDNA encoding an S-locus specific glycoprotein from Brassica oleracea plants containing the S5 self-incompatibility allele.Mol Gen Genet. 1990 Feb;220(3):409-13 PMID: 2338939
-
The S-locus specific glycoproteins of Brassica accumulate in the cell wall of developing stigma papillae.Dev Biol. 1989 Aug;134(2):462-72 PMID: 2472986
-
Self-incompatibility genes of Brassica oleracea: Expression, isolation, and structure.Proc Natl Acad Sci U S A. 1988 Aug;85(15):5551-5 PMID: 16593966
-
A homozygous S genotype of Brassica oleracea expresses two S-like genes.Mol Gen Genet. 1989 Jul;218(1):112-7 PMID: 2550759
-
Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
-
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.Anal Biochem. 1983 Jul 1;132(1):6-13 PMID: 6312838
-
Messenger RNA for G1 protein of French bean seeds: Cell-free translation and product characterization.Proc Natl Acad Sci U S A. 1978 Jul;75(7):3196-200 PMID: 16592543
-
A highly conserved Brassica gene with homology to the S-locus-specific glycoprotein structural gene.Plant Cell. 1989 Feb;1(2):249-58 PMID: 2535545
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: product@genelibs.com
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
