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PMID: 17065441 Published · ppublish English Comparative Study Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

A large-conductance calcium-selective mechanotransducer channel in mammalian cochlear hair cells.

The Journal of neuroscience : the official journal of the Society for Neuroscience ·Vol. 26 ·No. 43 ·2006-10-25 ·Pages 10992-1000

Beurg M, Evans MG, Hackney CM, Fettiplace R

Abstract

Sound stimuli are detected in the cochlea by opening of hair cell mechanotransducer (MT) channels, one of the few ion channels not yet conclusively identified at a molecular level. To define their performance in situ, we measured MT channel properties in inner hair cells (IHCs) and outer hair cells (OHCs) at two locations in the rat cochlea tuned to different characteristic frequencies (CFs). The conductance (in 0.02 mM calcium) of MT channels from IHCs was estimated as 260 pS at both low-frequency and mid-frequency positions, whereas that from OHCs increased with CFs from 145 to 210 pS. The combination of MT channel conductance and tip link number, assayed from scanning electron micrographs, accounts for variation in whole-cell current amplitude for OHCs and its invariance for IHCs. Channels from apical IHCs and OHCs having a twofold difference in unitary conductance were both highly calcium selective but were distinguishable by a small but significant difference in calcium permeability and in their response to lowering ionic strength. The results imply that the MT channel has properties possessed by few known candidates, and its diversity suggests expression of multiple isoforms.

MeSH Terms
Acoustic Stimulation/methods Animals Calcium/pharmacology,physiology Cochlea/drug effects,physiology,ultrastructure Hair Cells, Auditory, Inner/drug effects,physiology,ultrastructure Hair Cells, Auditory, Outer/drug effects,physiology,ultrastructure Large-Conductance Calcium-Activated Potassium Channels/physiology,ultrastructure Mechanoreceptors/physiology,ultrastructure Rats Rats, Sprague-Dawley
Chemicals
Large-Conductance Calcium-Activated Potassium Channels Calcium
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Beurg Maryline
Equipe Associée 3665 Université Victor Segalen Bordeaux 2, Institut National de la Santé et de la Recherche Médicale, Unité 587, Hôpital Pellegrin, 33076 Bordeaux, France.
Evans Michael G
Hackney Carole M
Fettiplace Robert
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Article Info
Journal
The Journal of neuroscience : the official journal of the Society for Neuroscience
Abbr.
J Neurosci
ISSN
1529-2401
DOI
10.1523/JNEUROSCI.2188-06.2006
Published
2006-10-25
Pages
10992-1000
Language
English
Region
United States
NLM ID
8102140
PMCID
PMC6674673
Subset
IM
Grants
NIDCD NIH HHS · R01 DC001362 · United States
NIDCD NIH HHS · R01 DC 01362 · United States
External Links
PubMed source DOI: 10.1523/JNEUROSCI.2188-06.2006 PMC Full Text View journal details
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