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PMID: 17054791 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Gene expression signatures of morphologically normal breast tissue identify basal-like tumors.

Breast cancer research : BCR ·Vol. 8 ·No. 5 ·2006-00-00 ·Pages R58

Finak G, Sadekova S, Pepin F, Hallett M, Meterissian S, Halwani F, Khetani K, Souleimanova M, Zabolotny B, Omeroglu A, Park M

Abstract

The role of the cellular microenvironment in breast tumorigenesis has become an important research area. However, little is known about gene expression in histologically normal tissue adjacent to breast tumor, if this is influenced by the tumor, and how this compares with non-tumor-bearing breast tissue. To address this, we have generated gene expression profiles of morphologically normal epithelial and stromal tissue, isolated using laser capture microdissection, from patients with breast cancer or undergoing breast reduction mammoplasty (n = 44). Based on this data, we determined that morphologically normal epithelium and stroma exhibited distinct expression profiles, but molecular signatures that distinguished breast reduction tissue from tumor-adjacent normal tissue were absent. Stroma isolated from morphologically normal ducts adjacent to tumor tissue contained two distinct expression profiles that correlated with stromal cellularity, and shared similarities with soft tissue tumors with favorable outcome. Adjacent normal epithelium and stroma from breast cancer patients showed no significant association between expression profiles and standard clinical characteristics, but did cluster ER/PR/HER2-negative breast cancers with basal-like subtype expression profiles with poor prognosis. Our data reveal that morphologically normal tissue adjacent to breast carcinomas has not undergone significant gene expression changes when compared to breast reduction tissue, and provide an important gene expression dataset for comparative studies of tumor expression profiles.

MeSH Terms
Adult Breast/physiology Breast Neoplasms/genetics Carcinoma, Ductal, Breast/genetics Epithelial Cells Female Gene Expression Profiling Humans Microdissection Middle Aged Stromal Cells
Authors & Affiliations
11 authors, click to expand affiliations / ORCID
Finak Greg
Molecular Oncology Group, McGill University Health Centre, 687 Pine Ave, West, H3A 1A1, Quebec, Canada.
Sadekova Svetlana
Pepin Francois
Hallett Michael
Meterissian Sarkis
Halwani Fawaz
Khetani Karim
Souleimanova Margarita
Zabolotny Brent
Omeroglu Atilla
Park Morag
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Article Info
Journal
Breast cancer research : BCR
Abbr.
Breast Cancer Res
ISSN
1465-542X
DOI
10.1186/bcr1608
Published
2006-00-00
Pages
R58
Language
English
Region
England
NLM ID
100927353
PMCID
PMC1779486
Subset
IM
External Links
PubMed source DOI: 10.1186/bcr1608 PMC Full Text View journal details
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