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PMID: 1705002 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Deletions in the SH2 domain of p60v-src prevent association with the detergent-insoluble cellular matrix.

Molecular and cellular biology ·Vol. 11 ·No. 3 ·1991-03-00 ·Pages 1207-13

Fukui Y, O'Brien MC, Hanafusa H

Abstract

p60v-src has been shown to associate with a detergent-insoluble cellular matrix containing cytoskeletal proteins, but p60c-src does not bind to this matrix. We analyzed the association of mutant src proteins with the matrix and found that mutants which lack an amino-terminal portion (residues 149 to 169) of the SH2 domain cannot bind to the matrix. Neither the SH3 region nor other portions of the SH2 region were required for association. We also tested protein kinase-defective mutants and chimeras of p60v-src and p60c-src. We found a strong correlation between the kinase activity of p60src and its association with the detergent-insoluble matrix. Double infection of kinase-defective and kinase-active mutants did not result in matrix binding of the kinase-defective src proteins. We also found that Tyr-416, the major site of autophosphorylation in p60v-src, was not required for matrix association.

Related Genes
c-src v-fps v-src v-yes
MeSH Terms
Amino Acid Sequence Animals Cell Line Cell Transformation, Neoplastic/metabolism Chick Embryo Cytoskeleton/metabolism DNA Mutational Analysis In Vitro Techniques Molecular Sequence Data Oncogene Protein pp60(v-src)/metabolism Protein Binding Protein-Tyrosine Kinases/metabolism Proto-Oncogene Proteins pp60(c-src)/metabolism Recombinant Fusion Proteins/metabolism Structure-Activity Relationship
Chemicals
Recombinant Fusion Proteins Protein-Tyrosine Kinases Oncogene Protein pp60(v-src) Proto-Oncogene Proteins pp60(c-src)
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Fukui Y
Rockefeller University, New York, New York 10021.
O'Brien M C
Hanafusa H
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42 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.11.3.1207-1213.1991
Published
1991-03-00
Pages
1207-13
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC369391
Subset
IM
Grants
NIAID NIH HHS · AI07233 · United States
NCI NIH HHS · CA44356 · United States
External Links
PubMed source DOI: 10.1128/mcb.11.3.1207-1213.1991 PMC Full Text View journal details
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