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PMID: 17043308 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Tsix-mediated epigenetic switch of a CTCF-flanked region of the Xist promoter determines the Xist transcription program.

Genes & development ·Vol. 20 ·No. 20 ·2006-10-15 ·Pages 2787-92

Navarro P, Page DR, Avner P, Rougeulle C

Abstract

Initiation of X inactivation depends on the coordinated expression of the sense/antisense pair Xist/Tsix. We show here that a precisely defined Xist promoter region flanked by CTCF is maintained by Tsix in a heterochromatic-like state in undifferentiated embryonic stem (ES) cells and shifts to a pseudoeuchromatic structure upon Tsix truncation. We further demonstrate that the epigenetic state of the Xist 5' region prior to differentiation predicts the efficiency of transcriptional machinery recruitment to the Xist promoter during differentiation. Our results provide mechanistic insights into the Tsix-mediated epigenetic regulation of Xist resulting in Xist promoter activation and initiation of X inactivation in differentiating ES cells.

MeSH Terms
Animals CCCTC-Binding Factor Cell Differentiation Chromatin/metabolism CpG Islands DNA-Binding Proteins/genetics Embryonic Stem Cells/cytology Epigenesis, Genetic Gene Expression Regulation, Developmental Histones/metabolism Male Mice Promoter Regions, Genetic RNA, Long Noncoding RNA, Untranslated/genetics,physiology Repressor Proteins/genetics Transcription, Genetic
Chemicals
CCCTC-Binding Factor Chromatin Ctcf protein, mouse DNA-Binding Proteins Histones RNA, Long Noncoding RNA, Untranslated Repressor Proteins Tsix transcript, mouse
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Navarro Pablo
Unité de Génétique Moléculaire Murine, Institut Pasteur 75724, Paris Cedex 15, France.
Page Damian R
Avner Philip
Rougeulle Claire
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.389006
Published
2006-10-15
Pages
2787-92
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC1619945
Subset
IM
External Links
PubMed source DOI: 10.1101/gad.389006 PMC Full Text View journal details
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