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PMID: 17015649 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Defining the Pseudomonas aeruginosa SOS response and its role in the global response to the antibiotic ciprofloxacin.

Journal of bacteriology ·Vol. 188 ·No. 20 ·2006-10-00 ·Pages 7101-10

Cirz RT, O'Neill BM, Hammond JA, Head SR, Romesberg FE

Abstract

Pseudomonas aeruginosa infections can be virtually impossible to eradicate, and the evolution of resistance during antibiotic therapy is a significant concern. In this study, we use DNA microarrays to characterize the global transcriptional response of P. aeruginosa to clinical-like doses of the antibiotic ciprofloxacin and also to determine the component that is regulated by LexA cleavage and the SOS response. We find that genes involved in virtually every facet of metabolism are down-regulated in response to ciprofloxacin. The LexA-controlled SOS regulon identified by microarray analysis includes only 15 genes but does include several genes that encode proteins involved in recombination and replication, including two inducible polymerases known to play a role in mutation and the evolution of antibiotic resistance in other organisms. The data suggest that the inhibition of LexA cleavage during therapy might help combat this pathogen by decreasing its ability to adapt and evolve resistance.

MeSH Terms
Anti-Bacterial Agents/pharmacology Bacterial Proteins/genetics Ciprofloxacin/pharmacology DNA-Directed DNA Polymerase/genetics Gene Expression Profiling Gene Expression Regulation, Bacterial Genes, Bacterial Oligonucleotide Array Sequence Analysis Pseudomonas aeruginosa/drug effects,genetics RNA, Bacterial/analysis,genetics RNA, Messenger/analysis,genetics Recombinases/genetics Regulon SOS Response, Genetics/genetics Serine Endopeptidases/genetics
Chemicals
Anti-Bacterial Agents Bacterial Proteins LexA protein, Bacteria RNA, Bacterial RNA, Messenger Recombinases Ciprofloxacin DNA-Directed DNA Polymerase Serine Endopeptidases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Cirz Ryan T
Department of Chemistry, The Scripps Research Institute, 10550 N. Torrey Pines Road, La Jolla, CA 92037, USA.
O'Neill Bryan M
Hammond Jennifer A
Head Steven R
Romesberg Floyd E
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.00807-06
Published
2006-10-00
Pages
7101-10
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC1636241
Subset
IM
Databases
GEO
GSE5443
External Links
PubMed source DOI: 10.1128/JB.00807-06 PMC Full Text View journal details
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