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PMID: 17008371 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Reversal of diet-induced insulin resistance with a single bout of exercise in the rat: the role of PTP1B and IRS-1 serine phosphorylation.

The Journal of physiology ·Vol. 577 ·No. Pt 3 ·2006-12-15 ·Pages 997-1007

Ropelle ER, Pauli JR, Prada PO, de Souza CT, Picardi PK, Faria MC, Cintra DE, Fernandes MF, Flores MB, Velloso LA, Saad MJ, Carvalheira JB

Abstract

Lifestyle interventions including exercise programmes are cornerstones in the prevention of obesity-related diabetes. In this study, we demonstrate that a single bout of exercise inhibits high-fat diet-induced insulin resistance. Diet-induced obesity (DIO) increased the expression and activity of the protein tyrosine phosphatase 1B (PTP1B) and attenuated insulin signalling in gastrocnemius muscle of rats, a phenomenon which was reversed by a single session of exercise. In addition, DIO was observed to lead to serine phosphorylation of insulin receptor substrate 1 (IRS-1), which was also reversed by exercise in muscle in parallel with a reduction in c-Jun N-terminal kinase (JNK) activity. Thus, acute exercise increased the insulin sensitivity during high-fat feeding in obese rats. Overall, these results provide new insights into the mechanism by which exercise restores insulin sensitivity.

MeSH Terms
Animals Dietary Fats/administration & dosage Dose-Response Relationship, Drug I-kappa B Proteins/antagonists & inhibitors Insulin/metabolism Insulin Receptor Substrate Proteins Insulin Resistance JNK Mitogen-Activated Protein Kinases/antagonists & inhibitors,metabolism Male Muscle, Skeletal/metabolism Obesity/etiology,metabolism,physiopathology Phosphoproteins/chemistry,metabolism Phosphorylation Physical Conditioning, Animal Protein Tyrosine Phosphatase, Non-Receptor Type 1 Protein Tyrosine Phosphatases/metabolism Rats Rats, Wistar Recovery of Function Serine/metabolism Signal Transduction Swimming
Chemicals
Dietary Fats I-kappa B Proteins Insulin Insulin Receptor Substrate Proteins Irs1 protein, rat Phosphoproteins Serine JNK Mitogen-Activated Protein Kinases Protein Tyrosine Phosphatase, Non-Receptor Type 1 Protein Tyrosine Phosphatases Ptpn1 protein, rat
Authors & Affiliations
12 authors, click to expand affiliations / ORCID
Ropelle Eduardo R
Departamento de Clínica, Médica, FCM-UNICAMP, Cidade Universitária Zeferino Vaz, Campinas, São Paulo, Brazil, 13081-970.
Pauli José R
Prada Patrícia O
de Souza Cláudio T
Picardi Paty K
Faria Marcel C
Cintra Dennys E
Fernandes Maria Fernanda de A
Flores Marcelo B
Velloso Lício A
Saad Mario J A
Carvalheira José B C
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Article Info
Journal
The Journal of physiology
Abbr.
J Physiol
ISSN
0022-3751
DOI
10.1113/jphysiol.2006.120006
Published
2006-12-15
Epub
2006-00-28
Pages
997-1007
Language
English
Region
England
NLM ID
0266262
PMCID
PMC1890392
Subset
IM
External Links
PubMed source DOI: 10.1113/jphysiol.2006.120006 PMC Full Text View journal details
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