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PMID: 17005703 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Production of infectious hepatitis C virus by well-differentiated, growth-arrested human hepatoma-derived cells.

Journal of virology ·Vol. 80 ·No. 20 ·2006-10-00 ·Pages 10253-7

Sainz B, Chisari FV

Abstract

Dimethyl sulfoxide (DMSO) has been shown to induce the differentiation of primary hepatocytes in vitro. When actively dividing poorly differentiated human hepatoma-derived (Huh7) cells were cultured in the presence of 1% DMSO, cells became cytologically differentiated and transitioned into a nondividing state, characterized by the induction of hepatocyte-specific genes. Moreover, these cells were highly permissive for acute hepatitis C virus (HCV) infection, and persistent long term infection of these cultures could also be achieved. As HCV naturally replicates in highly differentiated nondividing human hepatocytes, this system may more accurately mimic the conditions under which HCV replicates in vivo than previous models using poorly differentiated rapidly dividing hepatoma cells.

MeSH Terms
Carcinoma, Hepatocellular Cell Differentiation Cell Division Cell Line, Tumor Dimethyl Sulfoxide/pharmacology Hepacivirus/growth & development Hepatocytes/cytology,virology Humans RNA, Viral/analysis Virus Replication
Chemicals
RNA, Viral Dimethyl Sulfoxide
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sainz Bruno
Department of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, SBR-10, La Jolla, CA 92037, USA.
Chisari Francis V
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.01059-06
Published
2006-10-00
Pages
10253-7
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC1617281
Subset
IM
Grants
NCI NIH HHS · R01 CA108304 · United States
NIAID NIH HHS · T32 AI007354 · United States
NIAID NIH HHS · AI 07354-15 · United States
NCI NIH HHS · R01 CA 108304 · United States
External Links
PubMed source DOI: 10.1128/JVI.01059-06 PMC Full Text View journal details
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