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PMID: 1698612 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

T cell precursor migration towards beta 2-microglobulin is involved in thymus colonization of chicken embryos.

The EMBO journal ·Vol. 9 ·No. 10 ·1990-10-00 ·Pages 3315-22

Dunon D, Kaufman J, Salomonsen J, Skjoedt K, Vainio O, Thiery JP, Imhof BA

Abstract

beta 2-microglobulin (beta 2m) attracts hemopoietic precursors from chicken bone marrow cells in vitro. The cell population responding to beta 2m increases during the second period of thymus colonization, which takes place at days 12-14 of incubation. The precursors from 13.5 day old embryos were isolated after migration towards beta 2m in vitro and shown to be able to colonize a 13 day old thymus in ovo, where they subsequently acquire thymocyte markers. In contrast these beta 2m responsive precursors did not colonize embryonic bursa, i.e. differentiate into B lymphocytes. During chicken embryogenesis, peaks of beta 2m transcripts and of free beta 2m synthesis can only be detected in the thymus. The peak of free beta 2m synthesis in the thymus and the increase of beta 2m responding bone marrow cells both occur concomitantly with the second wave of thymus colonization in chicken embryo, facts which suggest that beta 2m mediated chemotaxis is involved in the second wave.

MeSH Terms
Animals Blotting, Northern Bone Marrow/immunology Chemotaxis, Leukocyte Chick Embryo Hematopoietic Stem Cells/immunology,physiology RNA/genetics,isolation & purification T-Lymphocytes/immunology,physiology Thymus Gland/embryology,immunology Transcription, Genetic beta 2-Microglobulin/genetics,immunology
Chemicals
beta 2-Microglobulin RNA
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Dunon D
Basel Institute for Immunology, Switzerland.
Kaufman J
Salomonsen J
Skjoedt K
Vainio O
Thiery J P
Imhof B A
References (42)
42 references, click to expand
  1. The chemotactic effect of mixtures of antibody and antigen on polymorphonuclear leucocytes.
    J Exp Med. 1962 Mar 1;115:453-66 PMID: 13872176
  2. Identification of a T3/T cell receptor complex in chickens.
    J Exp Med. 1986 Jul 1;164(1):375-80 PMID: 2425031
  3. Various rat adult tissues express only one major mRNA species from the glyceraldehyde-3-phosphate-dehydrogenase multigenic family.
    Nucleic Acids Res. 1985 Mar 11;13(5):1431-42 PMID: 2987824
  4. Isolation of RNA using guanidinium salts.
    Methods Enzymol. 1987;152:219-27 PMID: 2443794
  5. B-G cDNA clones have multiple small repeats and hybridize to both chicken MHC regions.
    Immunogenetics. 1989;30(6):440-51 PMID: 2592020
  6. A molecular map of the chicken major histocompatibility complex: the class II beta genes are closely linked to the class I genes and the nucleolar organizer.
    EMBO J. 1988 Sep;7(9):2775-85 PMID: 3141149
  7. Comparison of three actin-coding sequences in the mouse; evolutionary relationships between the actin genes of warm-blooded vertebrates.
    J Mol Evol. 1986;23(1):11-22 PMID: 3084797
  8. Thymotaxin: a thymic epithelial peptide chemotactic for T-cell precursors.
    Proc Natl Acad Sci U S A. 1988 Oct;85(20):7699-703 PMID: 3051002
  9. Establishment of cell lines from somite stage mouse embryos and expression of major histocompatibility class I genes in these cells.
    J Immunol. 1988 Jun 15;140(12):4378-87 PMID: 2453581
  10. Monoclonal antibodies against chicken Bu-1a and Bu-1b alloantigens.
    Hybridoma. 1988 Feb;7(1):41-8 PMID: 2453446
  11. The chicken erythrocyte-specific MHC antigen. Characterization and purification of the B-G antigen by monoclonal antibodies.
    Immunogenetics. 1987;25(6):373-82 PMID: 2439446
  12. Mouse major histocompatibility class I gene expression begins at midsomite stage and is inducible in earlier-stage embryos by interferon.
    Proc Natl Acad Sci U S A. 1985 Apr;82(8):2427-31 PMID: 2581247
  13. Characterization of rat T cell precursors sorted by chemotactic migration toward thymotaxin.
    Cell. 1989 Mar 24;56(6):1073-83 PMID: 2564314
  14. Autocrine induction of collagenase by serum amyloid A-like and beta 2-microglobulin-like proteins.
    Science. 1989 Feb 3;243(4891):655-7 PMID: 2536953
  15. Thymotaxin, a chemotactic protein, is identical to beta 2-microglobulin.
    Science. 1989 Nov 10;246(4931):803-6 PMID: 2683083
  16. Analysis of the first two waves of thymus homing stem cells and their T cell progeny in chick-quail chimeras.
    J Exp Med. 1989 Aug 1;170(2):543-57 PMID: 2666562
  17. Bu-1 antigen expression as a marker for B cell precursors in chicken embryos.
    Eur J Immunol. 1989 Feb;19(2):239-43 PMID: 2649381
  18. Migration of putative progenitor T cells in response to thymus-derived chemotactic factors.
    Cell Immunol. 1989 May;120(2):301-13 PMID: 2785857
  19. Isolation and characterization of chicken and turkey beta 2-microglobulin.
    Mol Immunol. 1986 Dec;23(12):1301-9 PMID: 3547090
  20. A chemotactic factor for rat thymocytes may regulate T-lymphocyte migration toward the thymic microenvironment.
    Cell Immunol. 1987 Aug;108(1):53-63 PMID: 3496976
  21. Ontogeny and tissue distribution of the chicken Bu-1a antigen.
    Immunology. 1987 Nov;62(3):463-70 PMID: 3499381
  22. The embryonic thymus produces chemotactic peptides involved in the homing of hemopoietic precursors.
    Cell. 1986 Mar 14;44(5):781-90 PMID: 3485015
  23. Evidence for a cyclic renewal of lymphocyte precursor cells in the embryonic chick thymus.
    Cell Differ. 1987 Nov;22(1):71-82 PMID: 3690675
  24. Homing of hemopoietic precursor cells to the embryonic thymus: characterization of an invasive mechanism induced by chemotactic peptides.
    J Cell Biol. 1986 Dec;103(6 Pt 2):2715-27 PMID: 3793754
  25. Clones of B lymphocytes in individual follicles of the bursa of Fabricius.
    Eur J Immunol. 1985 Jan;15(1):83-7 PMID: 3871398
  26. Ability of polymorphonuclear leukocytes to orient in gradients of chemotactic factors.
    J Cell Biol. 1977 Nov;75(2 Pt 1):606-16 PMID: 264125
  27. Beta 2-microglobulin deficient mice lack CD4-8+ cytolytic T cells.
    Nature. 1990 Apr 19;344(6268):742-6 PMID: 2139497
  28. Genetic analysis of a Drosophila neural cell adhesion molecule: interaction of fasciclin I and Abelson tyrosine kinase mutations.
    Cell. 1990 Feb 23;60(4):565-75 PMID: 2406026
  29. Seeding of the 10-day mouse embryo thymic rudiment by lymphocyte precursors in vitro.
    J Immunol. 1981 Jun;126(6):2310-6 PMID: 6971895
  30. Structure of wild-type and mutant mouse beta 2-microglobulin genes.
    Cell. 1982 Jun;29(2):661-9 PMID: 6180834
  31. The small subunit of HL-A antigens is beta 2-microglobulin.
    J Exp Med. 1973 Dec 1;138(6):1608-12 PMID: 4128442
  32. Isolation and properties of a low molecular weight beta-2-globulin occurring in human biological fluids.
    J Biol Chem. 1968 Aug 10;243(15):4095-103 PMID: 4175239
  33. Chemical properties of two antigens controlled by the major histocompatibility complex of the chicken.
    J Biol Chem. 1976 Sep 10;251(17):5391-6 PMID: 956191
  34. Multiple mRNA species with distinct 3' termini are transcribed from the beta 2-microglobulin gene.
    Nature. 1983 Mar 31-Apr 6;302(5907):449-52 PMID: 6188058
  35. Ontogeny of primary lymphoid organs and lymphoid stem cells.
    Am J Anat. 1984 Jul;170(3):261-99 PMID: 6383002
  36. Monoclonal antibodies against chicken lymphocyte surface antigens.
    Hybridoma. 1983;2(3):287-96 PMID: 6332057
  37. Isolation of molecules recognized by monoclonal antibodies and antisera: the solid phase immunoisolation technique.
    Anal Biochem. 1984 Feb;136(2):458-64 PMID: 6721145
  38. In vitro migration of avian hemopoietic cells to the thymus: preliminary characterization of a chemotactic mechanism.
    Cell Differ. 1983 Sep;13(1):1-24 PMID: 6627399
  39. Lymphoid stem cell homing to the early thymic primordium of the avian embryo.
    Eur J Immunol. 1980 Aug;10(8):620-7 PMID: 6995136
  40. In vitro migration of potential hemopoietic precursors from the murine fetus.
    Thymus. 1981 Jun;3(1):1-7 PMID: 7196609
  41. Demonstration of a cyclic renewal of the lymphocyte precursor cells in the quail thymus during embryonic and perinatal life.
    J Immunol. 1982 Nov;129(5):1869-77 PMID: 7119436
  42. Selective migration of 'null' cells towards a thymus factor in vitro.
    Thymus. 1981 Nov;3(4-5):187-94 PMID: 7314204
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1990-10-00
Pages
3315-22
Language
English
Region
England
NLM ID
8208664
PMCID
PMC552068
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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