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PMID: 16943567 Published · ppublish English Journal Article

Quantitative real-time RT-PCR of disseminated tumor cells in combination with immunomagnetic cell enrichment.

Molecular biotechnology ·Vol. 34 ·No. 1 ·2006-09-00 ·Pages 15-27

Lankiewicz S, Rivero BG, Bócher O

Abstract

Detection of disseminated tumor cells in the blood circulation is important in assessing tumor progression. The objective of this examination was to develop a highly specific and sensitive quantitative realtime reverse transcriptase-polymerase chain reaction (qRT-PCR) assay for the detection of relevant tumor-associated transcripts in patients' blood. The qRT-PCR assays detect the human epidermal growth factor receptor 2 (HER2) and CK20 transcripts of two tumor cells spiked into 5 mL of blood after an immunomagnetic tumor cell enrichment. Furthermore, the HER2 assay is only specific when enrichment is included. This procedure is a useful alternative to fluorescence in situ hybridization and immunocytochemistry for gene alteration analysis in human tumors. The analysis of the studied molecular markers of tumor cells in blood may be useful in the detection of disseminated tumor cells as well as for monitoring treatment response, early detection of relapse, and for stratification of patients with carcinoma.

MeSH Terms
Biomarkers, Tumor/analysis,genetics Breast Neoplasms/diagnosis,genetics,metabolism,pathology Cell Line Computer Systems Humans Immunomagnetic Separation/methods Neoplasm Invasiveness Neoplasm Proteins/analysis,genetics Reverse Transcriptase Polymerase Chain Reaction/methods
Chemicals
Biomarkers, Tumor Neoplasm Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lankiewicz Silke
AdnaGen AG, Ostpassage 7, 30583 Langenhagen, Germany. sl@adnagen.com
Rivero Bertha Gutierrez
Bócher Oliver
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Article Info
Journal
Molecular biotechnology
Abbr.
Mol Biotechnol
ISSN
1073-6085
DOI
10.1385/MB:34:1:15
Email
sl@adnagen.com
Published
2006-09-00
Pages
15-27
Language
English
Region
United States
NLM ID
9423533
Subset
IM
External Links
PubMed source DOI: 10.1385/MB:34:1:15 View journal details
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