Dendritic cells are the principal cells in mouse spleen bearing immunogenic fragments of foreign proteins.
We monitored the APC function of cells taken from the spleen and peritoneal cavity of mice that had been given protein antigens via the intravenous or intraperitoneal routes. Using the mAb 33D1 and N418 to negatively and positively select dendritic cells, we obtained evidence that dendritic cells are the main cell type in spleen that carries the protein in a form that is immunogenic for antigen-specific T cells. In vivo pulsed macrophages were not immunogenic and did not appear capable of transferring peptide fragments to dendritic cells.
15 references, click to expand
-
The interaction of soluble horseradish peroxidase with mouse peritoneal macrophages in vitro.J Cell Biol. 1972 Oct;55(1):186-204 PMID: 4347251
-
Contribution of dendritic cells to stimulation of the murine syngeneic mixed leukocyte reaction.J Exp Med. 1980 May 1;151(5):1196-212 PMID: 6445399
-
MHC class II antigen-bearing dendritic cells in pulmonary tissues of the rat. Regulation of antigen presentation activity by endogenous macrophage populations.J Exp Med. 1988 Feb 1;167(2):262-74 PMID: 3162253
-
Dendritic cells and the initiation of contact sensitivity to fluorescein isothiocyanate.Immunology. 1986 Dec;59(4):509-14 PMID: 3100437
-
Production of hapten-specific T cell hybridomas and their use to study the effect of ultraviolet B irradiation on the development of contact hypersensitivity.J Immunol. 1989 Dec 15;143(12):3867-72 PMID: 2480375
-
Presentation of exogenous protein antigens by dendritic cells to T cell clones. Intact protein is presented best by immature, epidermal Langerhans cells.J Exp Med. 1989 Mar 1;169(3):1169-78 PMID: 2522497
-
Characterization of sheep afferent lymph dendritic cells and their role in antigen carriage.J Exp Med. 1989 Oct 1;170(4):1285-301 PMID: 2794860
-
The cell surface of mouse dendritic cells: FACS analyses of dendritic cells from different tissues including thymus.Cell Immunol. 1989 Jan;118(1):108-25 PMID: 2910499
-
Antigen-presenting function of the macrophage.Annu Rev Immunol. 1984;2:395-428 PMID: 6242349
-
Intrathymic presentation of circulating non-MHC antigens by medullary dendritic cells. An antigen-dependent microenvironment for T cell differentiation.J Exp Med. 1986 Feb 1;163(2):231-46 PMID: 3484777
-
Correlation of T cell receptor V beta gene family with MHC restriction.J Exp Med. 1987 Aug 1;166(2):583-8 PMID: 3598467
-
Murine epidermal Langerhans cells mature into potent immunostimulatory dendritic cells in vitro.J Exp Med. 1985 Mar 1;161(3):526-46 PMID: 3871837
-
The distinct leukocyte integrins of mouse spleen dendritic cells as identified with new hamster monoclonal antibodies.J Exp Med. 1990 May 1;171(5):1753-71 PMID: 2185332
-
Antigen recognition by H-2-restricted T cells. I. Cell-free antigen processing.J Exp Med. 1983 Aug 1;158(2):303-16 PMID: 6193218
-
Identification of a novel cell type in peripheral lymphoid organs of mice. II. Functional properties in vitro.J Exp Med. 1974 Feb 1;139(2):380-97 PMID: 4589990
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