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PMID: 169269 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The permeability of muscle capillaries to horseradish peroxidase.

The Journal of cell biology ·Vol. 66 ·No. 3 ·1975-09-00 ·Pages 531-55

Williams MC, Wissig SL

Abstract

The main objective of this study was to determine the pathways by which horseradish peroxidase (HRP) can cross the endothelium of muscle capillaries. Specimens of mouse diaphragm were fixed for cytochemical analysis at various intervals after intervenous injection of 0.5 mg HRP, at 4 min after intervenous injection of varied amounts of HRP, and at 4 min after intervenous injections in various volumes of isotonic NaCl. Our findings indicate that endothelial junctions serve as a barrier which may allow passage of very limited amounts of HRP. They also suggest that endothelial vesicles transfer HRP from the capillary lumen to the pericapillary interstitium as well as in the reverse direction. Increasing the volume of solution injected to approximately 30% of total blood volume did not increase the amount of HRP that left the capillary lumen. Our results with HRP do not provide clearcut evidence that endothelial junctions are the site of the small pore.

MeSH Terms
Animals Basement Membrane/ultrastructure Capillaries/ultrastructure Capillary Permeability Cell Membrane/ultrastructure Diaphragm/blood supply Dose-Response Relationship, Drug Endothelium/ultrastructure Horseradish Peroxidase/metabolism Inclusion Bodies/ultrastructure Intercellular Junctions/physiology,ultrastructure Macrophages/ultrastructure Mice Mice, Inbred C3H Mice, Inbred Strains Microcirculation Muscles/blood supply Peroxidases/metabolism Time Factors
Chemicals
Horseradish Peroxidase Peroxidases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Williams M C
Wissig S L
References (43)
43 references, click to expand
  1. Transcapillary efflux of gamma globulin in rabbit skeletal muscle.
    Microvasc Res. 1975 Jan;9(1):119-26 PMID: 47141
  2. Permeability of muscle capillaries to exogenous myoglobin.
    J Cell Biol. 1973 May;57(2):424-52 PMID: 4696549
  3. Lymph flow and lymph protein concentration in the skin and muscle of the rabbit hind limb.
    J Physiol. 1973 Dec;235(2):477-92 PMID: 4763999
  4. Transport of proteins across normal cerebral arterioles.
    J Comp Neurol. 1973 Nov 1;152(1):17-44 PMID: 4765853
  5. Editorial: Filtration as the main mechanism of overall transcapillary protein escape from the plasma.
    Microvasc Res. 1974 May;7(3):i-iv PMID: 4829273
  6. Increased transcapillary escape rate of albumin, IgG, and IgM after plasma volume expansion.
    Am J Physiol. 1974 Aug;227(2):245-50 PMID: 4852404
  7. A low-viscosity epoxy resin embedding medium for electron microscopy.
    J Ultrastruct Res. 1969 Jan;26(1):31-43 PMID: 4887011
  8. Horseradish peroxidase in plasma studied by gel filtration.
    Histochemie. 1971;28(1):16-22 PMID: 5002924
  9. Studies on aortic intima. I. Structure and permeability of rat thoracic aortic intima.
    Am J Pathol. 1972 Feb;66(2):241-64 PMID: 5009972
  10. Physicochemical effects of aldehydes on the human erythrocyte.
    J Cell Biol. 1972 Jun;53(3):809-18 PMID: 5028261
  11. Mixing time and blood in normal and traumatized mice.
    Acta Chir Scand. 1971;137(2):107-11 PMID: 5149485
  12. Hemoglobin as a tracer in hemodynamic pulmonary edema.
    Science. 1969 Dec 26;166(3913):1643-6 PMID: 5360588
  13. The transport of macromolecules across the blood-lymph barrier. Influence of capillary pressure on macromolecular composition of lymph.
    Bibl Anat. 1969;10:228-33 PMID: 5407368
  14. Transport of large molecules from plasma to interstitial fluid and lymph in dogs.
    Am J Physiol. 1970 Dec;219(6):1595-605 PMID: 5485678
  15. A heme-peptide as an ultrastructural tracer.
    J Histochem Cytochem. 1970 Dec;18(12):911-3 PMID: 5502359
  16. Permeability of vasa nervorum and perineurium in mouse sciatic nerve studied by fluorescence and electron microscopy.
    J Neuropathol Exp Neurol. 1971 Jan;30(1):105-19 PMID: 5542492
  17. Studies on blood capillaries. I. General organization of blood capillaries in muscle.
    J Cell Biol. 1968 May;37(2):244-76 PMID: 5656394
  18. Intestinal capillaries. I. Permeability to peroxidase and ferritin.
    J Cell Biol. 1969 Apr;41(1):33-58 PMID: 5775791
  19. Distribution of peroxisomes (microbodies) in the nephron of the rat: a cytochemical study.
    J Cell Biol. 1969 Aug;42(2):501-18 PMID: 5792337
  20. Fine structures of capillary and endocapillary layer as revealed by ruthenium red.
    Fed Proc. 1966 Nov-Dec;25(6):1773-83 PMID: 5927412
  21. The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique.
    J Histochem Cytochem. 1966 Apr;14(4):291-302 PMID: 5962951
  22. The ultrastructural basis of capillary permeability studied with peroxidase as a tracer.
    J Cell Biol. 1967 Oct;35(1):213-36 PMID: 6061717
  23. Capillary permeability to macromolecules.
    Circ Res. 1955 Nov;3(6):594-603 PMID: 13270374
  24. Passage of dextran molecules across the blood-lymph barrier.
    Acta Chir Scand Suppl. 1956;211:1-84 PMID: 13326154
  25. Capillary permeability to macromolecules: stretched pore phenomenon.
    Am J Physiol. 1957 Aug;190(2):189-93 PMID: 13458438
  26. Passage of solid spherical particles across the blood-lymph barrier.
    Acta Physiol Scand. 1960 Dec 30;50:287-93 PMID: 13709135
  27. Blood capillaries of the heart and other organs.
    Circulation. 1961 Aug;24:368-88 PMID: 13732173
  28. STUDIES ON HEART LYMPH. II. CAPILLARY PERMEABILITY OF THE DOG'S HEART, USING DEXTRAN AS A TEST SUBSTANCE.
    Acta Physiol Scand. 1964 Nov;62:218-23 PMID: 14240444
  29. TRANSPORT OF LARGE MOLECULES ACROSS CAPILLARY WALLS.
    Physiologist. 1964 Feb;60:13-28 PMID: 14119009
  30. Improvements in epoxy resin embedding methods.
    J Biophys Biochem Cytol. 1961 Feb;9:409-14 PMID: 13764136
  31. A SIMPLIFIED LEAD CITRATE STAIN FOR USE IN ELECTRON MICROSCOPY.
    J Cell Biol. 1965 May;25:407-8 PMID: 14287192
  32. CHANGES IN THE ELECTROPHORETIC PATTERN IN LYMPH AND SERUM IN EXPERIMENTAL BURNS.
    J Clin Invest. 1943 Jul;22(4):627-33 PMID: 16695045
  33. The role of mucopolysaccharides in vesicle architecture and endothelial transport. An electron microscope study of myocardial blood vessels.
    J Cell Biol. 1972 Jan;52(1):198-206 PMID: 4108322
  34. Demonstration of cytochrome oxidase activity with diaminobenzidine. A biochemical and electron microscopic study.
    J Histochem Cytochem. 1972 Aug;20(8):583-9 PMID: 4114592
  35. Nature of the endoendothelial layer as demonstrated by ruthenium red.
    Exp Cell Res. 1970 Mar;59(3):491-2 PMID: 4190850
  36. Exogenous cytochrome c as an ultrastructural tracer.
    J Histochem Cytochem. 1969 Nov;17(11):751-3 PMID: 4195388
  37. Nondroplet ultrastructural demonstration of cytochrome oxidase activity with a polymerizing osmiophilic reagent, diaminobenzidine (DAB).
    J Cell Biol. 1968 Jul;38(1):1-14 PMID: 4300067
  38. Effects of hypophysectomy and TSH replacement on the ultrastructural localization of thyroperoxidase.
    Endocrinology. 1974 Aug;95(2):421-33 PMID: 4368648
  39. The functional ultrastructure of the blood-lymph barrier. Computer analysis of data from dog heart-lymph experiments using theoretical models.
    Acta Physiol Scand Suppl. 1972;374:1-30 PMID: 4506004
  40. The permeability of the alveolar-capillary membrane to ultrastructural protein tracers.
    Ann N Y Acad Sci. 1974;221:238-43 PMID: 4522551
  41. Studies on protein passage through arterial endothelium. I. Structural correlates of permeability in rat arterial endothelium.
    Lab Invest. 1973 Jun;28(6):672-7 PMID: 4576804
  42. Regional vascular volume and extravascular accumulation of labeled protein during plasma volume expansion.
    Am J Physiol. 1973 Mar;224(3):699-704 PMID: 4691289
  43. Studies on protein passage through arterial endothelium. II. Regional differences in permeability to fine structural protein tracers in arterial endothelium of normotensive rat.
    Lab Invest. 1973 Jun;28(6):678-85 PMID: 4714151
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.66.3.531
Published
1975-09-00
Pages
531-55
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2109454
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.66.3.531 PMC Full Text View journal details
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