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PMID: 16923916 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Superoxide dismutase B gene (sodB)-deficient mutants of Francisella tularensis demonstrate hypersensitivity to oxidative stress and attenuated virulence.

Journal of bacteriology ·Vol. 188 ·No. 17 ·2006-09-00 ·Pages 6443-8

Bakshi CS, Malik M, Regan K, Melendez JA, Metzger DW, Pavlov VM, Sellati TJ

Abstract

A Francisella tularensis live vaccine strain mutant (sodB(Ft)) with reduced Fe-superoxide dismutase gene expression was generated and found to exhibit decreased sodB activity and increased sensitivity to redox cycling compounds compared to wild-type bacteria. The sodB(Ft) mutant also was significantly attenuated for virulence in mice. Thus, this study has identified sodB as an important F. tularensis virulence factor.

MeSH Terms
Animals Bacterial Proteins/genetics Bacterial Vaccines/genetics Francisella tularensis/enzymology,pathogenicity,physiology Gene Deletion Hydrogen Peroxide/pharmacology Mice Mice, Inbred BALB C Mice, Inbred C57BL Oxidative Stress Paraquat/pharmacology Superoxide Dismutase/genetics Tularemia/microbiology Vaccines, Attenuated/genetics Virulence
Chemicals
Bacterial Proteins Bacterial Vaccines Vaccines, Attenuated Hydrogen Peroxide SodB protein, Bacteria Superoxide Dismutase Paraquat
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Bakshi Chandra Shekhar
Center for Immunology and Microbial Disease, Albany Medical College, 47 New Scotland Avenue, Albany, NY 12208-3479, USA.
Malik Meenakshi
Regan Kevin
Melendez J Andres
Metzger Dennis W
Pavlov Vitaly M
Sellati Timothy J
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.00266-06
Published
2006-09-00
Pages
6443-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC1595384
Subset
IM
Grants
NIAID NIH HHS · P01 AI056320 · United States
External Links
PubMed source DOI: 10.1128/JB.00266-06 PMC Full Text View journal details
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