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PMID: 16897177 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Glycomic studies of Drosophila melanogaster embryos.

Glycoconjugate journal ·Vol. 23 ·No. 5-6 ·2006-07-00 ·Pages 345-54

North SJ, Koles K, Hembd C, Morris HR, Dell A, Panin VM, Haslam SM

Abstract

With the complete genome sequence of Drosophila melanogaster defined a systematic approach towards understanding the function of glycosylation has become possible. Structural assignment of the entire Drosophila glycome during specific developmental stages could provide information that would shed further light on the specific roles of different glycans during development and pinpoint the activity of certain glycosyltransferases and other glycan biosynthetic genes that otherwise might be missed through genetic analyses. In this paper the major glycoprotein N- and O-glycans of Drosophila embryos are described as part of our initial undertaking to characterize the glycome of Drosophila melanogaster. The N-glycans are dominated by high mannose and paucimannose structures. Minor amounts of mono-, bi- and tri-antennary complex glycans were observed with GlcNAc and Galbeta1-4GlcNAc non-reducing end termini. O-glycans were restricted to the mucin-type core 1 Galbeta1-3GalNAc sequence.

MeSH Terms
Animals Carbohydrate Sequence Drosophila melanogaster/chemistry,embryology Embryo, Nonmammalian/chemistry Glycoproteins/chemistry Molecular Sequence Data Polysaccharides/chemistry
Chemicals
Glycoproteins Polysaccharides
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
North Simon J
Division of Molecular Biosciences, Faculty of Life Sciences, Imperial College, London, SW7 2AZ, UK.
Koles Kate
Hembd Caleb
Morris Howard R
Dell Anne
Panin Vladislav M
Haslam Stuart M
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Article Info
Journal
Glycoconjugate journal
Abbr.
Glycoconj J
ISSN
0282-0080
DOI
10.1007/s10719-006-6693-4
Published
2006-07-00
Pages
345-54
Language
English
Region
United States
NLM ID
8603310
Subset
IM
Grants
NIGMS NIH HHS · R01 GM069952 · United States
Wellcome Trust · United Kingdom
External Links
PubMed source DOI: 10.1007/s10719-006-6693-4 View journal details
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