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PMID: 16880396 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Stimulation of phosphatidylinositol kinase type I-mediated phosphatidylinositol (4,5)-bisphosphate synthesis by AP-2mu-cargo complexes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 103 ·No. 32 ·2006-08-08 ·Pages 11934-9

Krauss M, Kukhtina V, Pechstein A, Haucke V

Abstract

Phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P(2)] is an important factor for a variety of cellular functions ranging from cell signaling to actin cytoskeletal dynamics and endocytic membrane traffic. Here, we have identified the clathrin adaptor complex AP-2 as a regulator of phosphatidylinositol 4-phosphate 5-kinase (PIPK)-mediated PI(4,5)P(2) synthesis. AP-2 directly interacts with the kinase core domain of type I PIPK isozymes via its mu2-subunit in vitro and in native protein extracts. Endocytic cargo protein binding to mu2 leads to a potent stimulation of PIPK activity. These data thus identify a positive feedback loop consisting of endocytic cargo proteins, AP-2mu, and PIPK type I which may provide a specific pool of PI(4,5)P(2) dedicated to clathrin/AP-2-dependent receptor internalization.

MeSH Terms
Adaptor Protein Complex 2/chemistry Adaptor Protein Complex mu Subunits/chemistry Animals COS Cells Chlorocebus aethiops Cytoskeleton/metabolism Feedback, Physiological Fibroblasts/metabolism HeLa Cells Humans Phosphatidylinositol 4,5-Diphosphate/chemistry Phosphotransferases (Alcohol Group Acceptor)/metabolism Protein Binding Protein Isoforms Transcription Factor AP-2/metabolism
Chemicals
Adaptor Protein Complex 2 Adaptor Protein Complex mu Subunits Phosphatidylinositol 4,5-Diphosphate Protein Isoforms Transcription Factor AP-2 adaptor protein complex 2, mu 1 subunit Phosphotransferases (Alcohol Group Acceptor) 1-phosphatidylinositol-4-phosphate 5-kinase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Krauss Michael
Institute of Chemistry and Biochemistry, Department of Membrane Biochemistry, Freie Universität Berlin, Takustrasse 6, 14195 Berlin, Germany.
Kukhtina Viktoria
Pechstein Arndt
Haucke Volker
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.0510306103
Published
2006-08-08
Epub
2006-00-31
Pages
11934-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC1567676
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.0510306103 PMC Full Text View journal details
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