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PMID: 168585 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Interaction of partially purified simian virus 40 T antigen with circular viral DNA molecules.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 5 ·1975-05-00 ·Pages 1960-4

Jessel D, Hudson J, Landau T, Tenen D, Livingston DM

Abstract

Mixing chromatographic fractions containing simian virus 40 (SV40) T antigen with SV40 [3H]-DNA I (double-stranded, circular, supercoiled) results in the conversion of the nucleic acid to a form that will bind to a nitrocellulose filter. Unlabeled SV40 DNA I successfully competes with this reaction. Under the conditions employed, the antigen-containing fractions bind a variety of circular, viral DNA molecules. Chromatography of the antigen in three systems reveals that the T immunoreactivity migrates with DNA binding activity. In a kinetic heat inactivation experiment, the antigenic reactivity disappears simultaneously with the DNA binding activity. The data indicate the presence of a discernible DNA binding activity in fractions containing T antigen and suggest that the T antigen is the DNA binding protein being measured.

MeSH Terms
Antigen-Antibody Reactions Antigens, Viral/isolation & purification Binding Sites Cell Line Cell Transformation, Neoplastic Chromatography, Ion Exchange Coliphages/analysis Complement Fixation Tests DNA, Circular DNA, Viral Drug Stability Escherichia coli/analysis Hot Temperature Humans Kinetics Macromolecular Substances Nucleic Acid Conformation Protein Binding Simian virus 40/analysis,immunology
Chemicals
Antigens, Viral DNA, Circular DNA, Viral Macromolecular Substances
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Jessel D
Hudson J
Landau T
Tenen D
Livingston D M
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23 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.5.1960
Published
1975-05-00
Pages
1960-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC432668
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.5.1960 PMC Full Text View journal details
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