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PMID: 16855026 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The mRNA export factor Sus1 is involved in Spt/Ada/Gcn5 acetyltransferase-mediated H2B deubiquitinylation through its interaction with Ubp8 and Sgf11.

Molecular biology of the cell ·Vol. 17 ·No. 10 ·2006-10-00 ·Pages 4228-36

Köhler A, Pascual-García P, Llopis A, Zapater M, Posas F, Hurt E, Rodríguez-Navarro S

Abstract

Sus1 acts in nuclear mRNA export via its association with the nuclear pore-associated Sac3-Thp1-Cdc31 complex. In addition, Sus1 plays a role in transcription through its interaction with the Spt/Ada/Gcn5 acetyltransferase (SAGA) complex. Here, we have analyzed function and interaction of Sus1 within the SAGA complex. We demonstrate that Sus1 is involved in the SAGA-dependent histone H2B deubiquitinylation and maintenance of normal H3 methylation levels. By deletion analyses, we show that binding of Sus1 to SAGA depends on the deubiquitinylating enzyme Ubp8 and Sgf11. Moreover, a stable subcomplex between Sus1, Sgf11, and Ubp8 could be dissociated from SAGA under high salt conditions. In vivo recruitment of Sus1 to the activated GAL1 promoter depends on Ubp8 and vice versa. In addition, histones coenrich during SAGA purification in a Sus1-Sgf11-Ubp8-dependent way. Interestingly, sgf11 deletion enhances the mRNA export defect observed in sus1delta cells. Thus, the Sus1-Sgf11-Ubp8 module could work at the junction between SAGA-dependent transcription and nuclear mRNA export.

MeSH Terms
Acetyltransferases/metabolism Active Transport, Cell Nucleus Endopeptidases/metabolism Galactosidases/genetics Gene Expression Regulation, Fungal Histones/metabolism Methylation Nuclear Proteins/genetics,metabolism Promoter Regions, Genetic RNA Transport RNA, Messenger/metabolism RNA-Binding Proteins/genetics,metabolism Saccharomyces cerevisiae/enzymology,genetics,metabolism Saccharomyces cerevisiae Proteins/genetics,metabolism Salts/chemistry Trans-Activators/metabolism Transcription Factors/genetics,metabolism Ubiquitin/metabolism
Chemicals
Histones Nuclear Proteins RNA, Messenger RNA-Binding Proteins SAGA complex, S cerevisiae SGF11 protein, S cerevisiae Saccharomyces cerevisiae Proteins Salts Sus1 protein, S cerevisiae Trans-Activators Transcription Factors Ubiquitin Acetyltransferases Galactosidases Endopeptidases UBP8 protein, S cerevisiae
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Köhler Alwin
Biochemie-Zentrum der Universität Heidelberg, INF328, D-69120, Heidelberg, Germany.
Pascual-García Pau
Llopis Ana
Zapater Meritxell
Posas Francesc
Hurt Ed
Rodríguez-Navarro Susana
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.e06-02-0098
Published
2006-10-00
Epub
2006-00-19
Pages
4228-36
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC1635344
Subset
IM
External Links
PubMed source DOI: 10.1091/mbc.e06-02-0098 PMC Full Text View journal details
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