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PMID: 1684688 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Immunoreactivity of cerebral amyloidosis is enhanced by protein denaturation treatments.

Acta neuropathologica ·Vol. 82 ·No. 4 ·1991-00-00 ·Pages 260-5

Doi-Yi R, Kitamoto T, Tateishi J

Abstract

We investigated paraffin-embedded brain sections from three patients with Gerstmann-Sträussler syndrome and three patients with Alzheimer's disease or senile dementia of Alzheimer type using anti-human prion protein antisera and anti-beta/A4 protein antisera after protein denaturation treatments. After incubation with guanidine-thiocyanate, trichloroacetate, and phenol, the immunoreactivity of kuru plaques and senile plaques was enhanced to the same level as the formic acid treatment. These treatments revealed small compact amyloid deposits, amyloid deposits surrounding the plaque cores, and diffuse plaques. Most of these chemicals changed the congophilia of both amyloids. It is possible that these treatments denature amyloid fibril proteins and break down the structure of amyloid fibrils, thus revealing buried epitopes.

MeSH Terms
Adolescent Aged Aged, 80 and over Alzheimer Disease/immunology,metabolism,pathology Amyloid beta-Peptides/immunology Cerebral Amyloid Angiopathy/immunology,pathology Female Gerstmann-Straussler-Scheinker Disease/immunology,metabolism,pathology Humans Immunohistochemistry Kuru/immunology,pathology Male Middle Aged Nerve Tissue Proteins/immunology Paraffin Embedding PrPSc Proteins Prions/immunology Protein Denaturation Solvents
Chemicals
Amyloid beta-Peptides Nerve Tissue Proteins PrPSc Proteins Prions Solvents
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Doi-Yi R
Department of Neuropathology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Kitamoto T
Tateishi J
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32 references, click to expand
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Article Info
Journal
Acta neuropathologica
Abbr.
Acta Neuropathol
ISSN
0001-6322
DOI
10.1007/BF00308810
Published
1991-00-00
Pages
260-5
Language
English
Region
Germany
NLM ID
0412041
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00308810 View journal details
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