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PMID: 16824137 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Review

Understanding and making use of human memory B cells.

Immunological reviews ·Vol. 211 ·2006-06-00 ·Pages 303-9

Lanzavecchia A, Bernasconi N, Traggiai E, Ruprecht CR, Corti D, Sallusto F

Abstract

The work of our laboratory has focused on the study of human memory B cells. Using an in vitro approach we dissected the triggering requirements of B cells and unveiled a distinct role for TLRs in the activation of naive versus memory B cells. Using an ex vivo approach we analyzed the dynamics of memory B cells and ASCs and the kinetics of serum antibodies during secondary immune responses and in steady state conditions and used these quantitative data to build up a model of serological memory. According to this model memory B cells behave as ;stem cells' capable of generating plasma cells and antibodies in an antigen-dependent as well as in an antigen-independent fashion. Finally we developed an efficient method to interrogate human memory B cells and to isolate human monoclonal antibodies. This method can be exploited for the production of neutralizing antibodies for serotherapy and for "analytic vaccinology".

MeSH Terms
Animals Antibodies, Monoclonal/immunology Antibody-Producing Cells/immunology B-Lymphocytes/immunology Humans Immunization, Secondary Immunologic Memory/immunology Lymphocyte Activation/immunology Mice Toll-Like Receptors/immunology Vaccination
Chemicals
Antibodies, Monoclonal Toll-Like Receptors
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Lanzavecchia Antonio
Institute for Research in Biomedicine, Bellinzona, Switzerland. lanzavecchia@irb.unisi.ch
Bernasconi Nadia
Traggiai Elisabetta
Ruprecht Claudia R
Corti Davide
Sallusto Federica
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Article Info
Journal
Immunological reviews
Abbr.
Immunol Rev
ISSN
0105-2896
DOI
10.1111/j.0105-2896.2006.00403.x
Email
lanzavecchia@irb.unisi.ch
Published
2006-06-00
Pages
303-9
Language
English
Region
England
NLM ID
7702118
PMCID
PMC7165660
Subset
IM
Grants
NIAID NIH HHS · U19-AI057266 · United States
External Links
PubMed source DOI: 10.1111/j.0105-2896.2006.00403.x PMC Full Text View journal details
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