Blocks in elongation and initiation of protein synthesis induced by interferon treatment in mouse L cells.

European journal of biochemistry ·Vol. 54 ·No. 1 ·1975-05-00 ·Pages 1-10

Content J, Lebleu B, Nudel U, Zilberstein A, Berissi H, Revel M

Abstract

Synthesis of polypeptide chains coded by exogenous messenger RNAs is inhibited in cell-free extracts from interferon-treated mouse L cells, due to a "deficiency" in some specific tRNA species. A detailed analysis shows that polypeptide chain elongation is blocked and incomplete chains are formed. After a few minutes, however, initiation of new polypeptide chains is also blocked. Messenger RNA still binds to ribosomes but initiator Met-tRNA(FMET) binding is inhibited. The block in initiation appears to be secondary to the block in elongation.

MeSH Terms

Animals Centrifugation, Density Gradient Clone Cells Electrophoresis, Polyacrylamide Gel Hemoglobins/biosynthesis Interferons/pharmacology Kinetics L Cells/drug effects,metabolism,ultrastructure Mengovirus Mice N-Formylmethionine Peptide Chain Elongation, Translational/drug effects Peptide Chain Initiation, Translational/drug effects Protein Biosynthesis RNA, Messenger/metabolism RNA, Transfer/metabolism Rabbits Ribosomes/metabolism Sodium Dodecyl Sulfate

Chemicals

Hemoglobins RNA, Messenger Sodium Dodecyl Sulfate N-Formylmethionine Interferons RNA, Transfer

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Content J

Lebleu B

Nudel U

Zilberstein A

Berissi H

Revel M

Article Info

Journal

European journal of biochemistry

Abbr.

Eur J Biochem

ISSN

0014-2956

DOI

10.1111/j.1432-1033.1975.tb04106.x

Published

1975-05-00

Pages

1-10

Language

English

Region

England

NLM ID

0107600

Subset

External Links

PubMed source DOI: 10.1111/j.1432-1033.1975.tb04106.x

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Blocks in elongation and initiation of protein synthesis induced by interferon treatment in mouse L cells.

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