Home LiteratureArticle Details
PMID: 16789160 Published · ppublish English Journal Article

Heteroduplex mapping of heat-resistant deletion mutants of bacteriophage t5.

Journal of virology ·Vol. 15 ·No. 5 ·1975-05-00 ·Pages 1276-80

Scheible PP, Rhoades M

Abstract

The bacteriophage T5 is known to spontaneously generate deletion mutants (st mutants) exhibiting enhanced resistance to heat inactivation in citrate buffer. A series of such mutants has been isolated and the deletions visualized by electron microscopy of heteroduplex molecules. The deletions are found to cluster in one region of the chromosome.

Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Scheible P P
Department of Biology, University of Virginia, Charlottesville, Virginia 22901.
Rhoades M
References (15)
15 references, click to expand
  1. Terminal repetition in the DNA of bacteriophage T5.
    J Mol Biol. 1972 Aug 21;69(2):187-200 PMID: 4560946
  2. A buoyant method for the determination of the superhelix density of closed circular DNA.
    J Mol Biol. 1971 Nov 28;62(1):1-19 PMID: 4333613
  3. Bacteriophage phi-X174 and related viruses.
    Prog Nucleic Acid Res Mol Biol. 1968;8:115-69 PMID: 4874231
  4. Heat-stable mutants of T5 phage. I. The physical properties of the phage and their DNA molecules.
    Virology. 1968 Nov;36(3):356-76 PMID: 4881031
  5. Heat-stable and density mutants of phages T1, T3 and T7.
    J Gen Virol. 1970 Oct;9(1):35-43 PMID: 4921715
  6. The chromosome of bacteriophage T5. II. Arrangement of the single-stranded DNA fragments in the T5 + and T5st(O) chromosomes.
    J Mol Biol. 1972 Feb 14;63(3):397-407 PMID: 5014926
  7. Analysis of the interruptions in bacteriophage T5 DNA.
    J Mol Biol. 1970 Feb 14;47(3):477-93 PMID: 5418167
  8. A physical map of the left arm of the lambda chromosome.
    J Mol Biol. 1971 Mar 14;56(2):425-8 PMID: 5551397
  9. Mapping of deletions and substitutions in heteroduplex DNA molecules of bacteriophage lambda by electron microscopy.
    Science. 1969 Mar 21;163(3873):1343-8 PMID: 5765116
  10. Density mutants of phage T5.
    Virology. 1962 Dec;18:576-81 PMID: 13954145
  11. Sedimentation rate as a measure of molecular weight of DNA.
    Biophys J. 1963 Jul;3:309-21 PMID: 14016998
  12. THE ARRANGEMENTS OF NUCLEOTIDE SEQUENCES IN T2 AND T5 BACTERIOPHAGE DNA MOLECULES.
    Biophys J. 1964 Mar;4:93-106 PMID: 14130440
  13. Mutation to heat resistance in coliphage T51.
    J Immunol. 1950 Apr;64(4):335-47 PMID: 15415601
  14. Deletion mutants of bacteriophage lambda. I. Isolation and initial characterization.
    J Mol Biol. 1971 Mar 14;56(2):369-84 PMID: 4323931
  15. Physical mapping of the early region of bacteriophage T7 DNA.
    J Mol Biol. 1973 Sep 15;79(2):249-65 PMID: 4586410
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
Published
1975-05-00
Pages
1276-80
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC354585
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com