Home LiteratureArticle Details
PMID: 16788206 Published · ppublish English Journal Article

GlnR-mediated regulation of nitrogen metabolism in Lactococcus lactis.

Journal of bacteriology ·Vol. 188 ·No. 13 ·2006-07-00 ·Pages 4978-82

Larsen R, Kloosterman TG, Kok J, Kuipers OP

Abstract

We show that the nitrogen regulatory protein GlnR of Lactococcus lactis represses transcription of the amtB-glnK, glnRA, and glnPQ operons. This likely occurs through a conserved DNA motif, 5'-TGTNA-7N-TNACAT-3', and takes place in response to extracellular glutamine and ammonium. GlnR-independent repression of amtB-glnK is mediated by the pleiotropic nitrogen regulator CodY.

MeSH Terms
Bacterial Proteins/genetics Base Sequence Gene Expression Regulation, Bacterial Glutamine Lactococcus lactis/genetics,growth & development,metabolism Molecular Sequence Data Nitrogen/metabolism Operon/genetics Quaternary Ammonium Compounds Trans-Activators/genetics
Chemicals
Bacterial Proteins Quaternary Ammonium Compounds Trans-Activators Glutamine Nitrogen
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Larsen Rasmus
Department of Molecular Genetics, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands.
Kloosterman Tomas G
Kok Jan
Kuipers Oscar P
References (30)
30 references, click to expand
  1. Regulation of nitrogen metabolism in Bacillus subtilis: vive la différence!
    Mol Microbiol. 1999 Apr;32(2):223-32 PMID: 10231480
  2. The glnKamtB operon. A conserved gene pair in prokaryotes.
    Trends Genet. 2000 Jan;16(1):11-4 PMID: 10637624
  3. Role of TnrA in nitrogen source-dependent repression of Bacillus subtilis glutamate synthase gene expression.
    J Bacteriol. 2000 Nov;182(21):5939-47 PMID: 11029411
  4. The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403.
    Genome Res. 2001 May;11(5):731-53 PMID: 11337471
  5. Functional analysis of 14 genes that constitute the purine catabolic pathway in Bacillus subtilis and evidence for a novel regulon controlled by the PucR transcription activator.
    J Bacteriol. 2001 Jun;183(11):3293-302 PMID: 11344136
  6. Pleiotropic transcriptional repressor CodY senses the intracellular pool of branched-chain amino acids in Lactococcus lactis.
    Mol Microbiol. 2001 Jun;40(5):1227-39 PMID: 11401725
  7. A higher-order background model improves the detection of promoter regulatory elements by Gibbs sampling.
    Bioinformatics. 2001 Dec;17(12):1113-22 PMID: 11751219
  8. A Gibbs sampling method to detect overrepresented motifs in the upstream regions of coexpressed genes.
    J Comput Biol. 2002;9(2):447-64 PMID: 12015892
  9. Transcriptome analysis and related databases of Lactococcus lactis.
    Antonie Van Leeuwenhoek. 2002 Aug;82(1-4):113-22 PMID: 12369183
  10. Roles of PucR, GlnR, and TnrA in regulating expression of the Bacillus subtilis ure P3 promoter.
    J Bacteriol. 2002 Nov;184(21):6060-4 PMID: 12374841
  11. Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box.
    Mol Microbiol. 2003 Jul;49(1):157-65 PMID: 12823818
  12. ArgR and AhrC are both required for regulation of arginine metabolism in Lactococcus lactis.
    J Bacteriol. 2004 Feb;186(4):1147-57 PMID: 14762010
  13. MicroPreP: a cDNA microarray data pre-processing framework.
    Appl Bioinformatics. 2003;2(4):241-4 PMID: 15130795
  14. Probing direct interactions between CodY and the oppD promoter of Lactococcus lactis.
    J Bacteriol. 2005 Jan;187(2):512-21 PMID: 15629923
  15. Substrate specificity and ionic regulation of GlnPQ from Lactococcus lactis. An ATP-binding cassette transporter with four extracytoplasmic substrate-binding domains.
    J Biol Chem. 2005 Jun 24;280(25):23785-90 PMID: 15795228
  16. A generally applicable validation scheme for the assessment of factors involved in reproducibility and quality of DNA-microarray data.
    BMC Genomics. 2005 May 20;6:77 PMID: 15907200
  17. The Lactococcus lactis CodY regulon: identification of a conserved cis-regulatory element.
    J Biol Chem. 2005 Oct 7;280(40):34332-42 PMID: 16040604
  18. Improved medium for lactic streptococci and their bacteriophages.
    Appl Microbiol. 1975 Jun;29(6):807-13 PMID: 16350018
  19. Nutritional Requirements in Multiple Auxotrophic Lactic Acid Bacteria: Genetic Lesions Affecting Amino Acid Biosynthetic Pathways in Lactococcus lactis, Enterococcus faecium, and Pediococcus acidilactici.
    Biosci Biotechnol Biochem. 1992 Jan;56(6):913-8 PMID: 27280812
  20. Relation of growth of Streptococcus lactis and Streptococcus cremoris to amino acid transport.
    J Bacteriol. 1988 Feb;170(2):700-7 PMID: 3123462
  21. Regulation of glutamine synthetase from Bacillus subtilis by divalent cations, feedback inhibitors, and L-glutamine.
    J Biol Chem. 1974 Jan 10;249(1):257-64 PMID: 4149044
  22. Plasmid complements of Streptococcus lactis NCDO 712 and other lactic streptococci after protoplast-induced curing.
    J Bacteriol. 1983 Apr;154(1):1-9 PMID: 6403500
  23. Gene inactivation in Lactococcus lactis: branched-chain amino acid biosynthesis.
    J Bacteriol. 1993 Jul;175(14):4383-90 PMID: 8331070
  24. Organization and regulation of genes for amino acid biosynthesis in lactic acid bacteria.
    FEMS Microbiol Rev. 1993 Sep;12(1-3):21-37 PMID: 8398216
  25. Autogenous regulation of the Bacillus subtilis glnRA operon.
    J Bacteriol. 1996 Apr;178(8):2450-4 PMID: 8636055
  26. TnrA, a transcription factor required for global nitrogen regulation in Bacillus subtilis.
    Proc Natl Acad Sci U S A. 1996 Aug 20;93(17):8841-5 PMID: 8799114
  27. A general system for generating unlabelled gene replacements in bacterial chromosomes.
    Mol Gen Genet. 1996 Nov 27;253(1-2):217-24 PMID: 9003306
  28. Expression of the Bacillus subtilis ureABC operon is controlled by multiple regulatory factors including CodY, GlnR, TnrA, and Spo0H.
    J Bacteriol. 1997 Sep;179(17):5494-501 PMID: 9287005
  29. Identification of a sodium chloride-regulated promoter in Lactococcus lactis by single-copy chromosomal fusion with a reporter gene.
    Mol Gen Genet. 1998 Apr;257(6):681-5 PMID: 9604892
  30. Nitrogen and oxygen regulation of Bacillus subtilis nasDEF encoding NADH-dependent nitrite reductase by TnrA and ResDE.
    J Bacteriol. 1998 Oct;180(20):5344-50 PMID: 9765565
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.00025-06
Published
2006-07-00
Pages
4978-82
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC1483007
Subset
IM
External Links
PubMed source DOI: 10.1128/JB.00025-06 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2