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PMID: 16766680 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

A novel class of small RNAs in mouse spermatogenic cells.

Genes & development ·Vol. 20 ·No. 13 ·2006-07-01 ·Pages 1709-14

Grivna ST, Beyret E, Wang Z, Lin H

Abstract

Small noncoding RNAs, including small interfering RNAs (siRNAs) and micro RNAs (miRNAs) of approximately 21 nucleotides (nt) in length, have emerged as potent regulators of gene expression at both transcriptional and post-transcriptional levels in diverse organisms. Here we report the identification of a novel class of small RNAs in the mouse male germline termed piwi-interacting RNAs (piRNAs). piRNAs are approximately 30 nt in length. They are expressed during spermatogenesis, mostly in spermatids. piRNAs are associated with MIWI, a spermatogenesis-specific PIWI subfamily member of the Argonaute protein family, and depend on MIWI for their biogenesis and/or stability. Furthermore, a subpopulation of piRNAs are associated with polysomes, suggesting their potential role in translational regulation.

MeSH Terms
Animals Argonaute Proteins Cloning, Molecular Genome Male Mice Protein Biosynthesis Proteins/genetics,metabolism RNA/classification,genetics,metabolism RNA, Small Interfering/genetics,metabolism Spermatids/physiology Spermatogenesis Testis/cytology,physiology
Chemicals
Argonaute Proteins Piwil1 protein, mouse Proteins RNA, Small Interfering RNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Grivna Shane T
Department of Cell Biology, Duke University, Durham, North Carolina 27710, USA.
Beyret Ergin
Wang Zhong
Lin Haifan
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.1434406
Published
2006-07-01
Epub
2006-00-09
Pages
1709-14
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC1522066
Subset
IM
Grants
NCI NIH HHS · T32 CA59365 · United States
NICHD NIH HHS · HD42012 · United States
NCI NIH HHS · T32 CA059365 · United States
NICHD NIH HHS · R01 HD042012 · United States
NICHD NIH HHS · R37 HD042012 · United States
External Links
PubMed source DOI: 10.1101/gad.1434406 PMC Full Text View journal details
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