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PMID: 1676616 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Polymorphic karyotypes in related Acremonium strains.

Current genetics ·Vol. 19 ·No. 2 ·1991-02-00 ·Pages 73-6

Walz M, Kück U

Abstract

A restriction fragment length polymorphism (RFLP) analysis was performed on six related Acremonium strains. With respect to the restriction fragment pattern, all strains of A. chrysogenum were indistinguishable from each other but showed distinctive differences from those of A. strictum, A. flavum and Cephalosporium polyvaleurum. Using pulsed-field gel electrophoresis, we obtained different chromosome patterns from most of the Acremonium strains. Remarkably, the pattern varies in three related A. chrysogenum strains which also differ in their rate of cephalosporin C biosynthesis. The electrophoretic karyotyping was confirmed by the location of rDNA genes on separate chromosomes. Our data indicate that chromosome translocations in industrial strains may be responsible for increased beta-lactam synthesis.

MeSH Terms

Acremonium/classification,genetics Blotting, Southern Electrophoresis, Agar Gel Karyotyping Polymorphism, Restriction Fragment Length

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Walz M

Lehrstuhl für Allgemeine Botanik, Ruhr-Universität Bochum, Federal Republic of Germany.

Kück U

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13 references, click to expand

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Article Info

Journal

Current genetics

Abbr.

Curr Genet

ISSN

0172-8083

DOI

10.1007/BF00326285

Published

1991-02-00

Pages

73-6

Language

English

Region

United States

NLM ID

8004904

Subset

External Links

PubMed source DOI: 10.1007/BF00326285 View journal details

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