Separation of antigens by immunological specificity: Use of cellulose-linked antibodies as immunosorbents.

The Biochemical journal ·Vol. 101 ·No. 3 ·1966-12-00 ·Pages 711-6

Gallop RG, Tozer BT, Stephen J, Smith H

Abstract

1. Immunosorbents were prepared by coupling activated aminocellulose with the gamma-globulin concentrates of antisera prepared against ovalbumin and human serum albumin. 2. The immunosorbents were low in solubility, but high in capacity for homologous antigens. 3. The high specificity of these immunosorbents was demonstrated by their use in fractionating various mixtures of fluorescent ovalbumin, (131)I-labelled human serum albumin, lysozyme and ribonuclease.

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Gallop R G

Microbiological Research Establishment, Porton Down, Wilts.

Tozer B T

Stephen J

Smith H

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15 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1010711

Published

1966-12-00

Pages

711-6

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1270177

External Links

PubMed source DOI: 10.1042/bj1010711 PMC Full Text View journal details

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Separation of antigens by immunological specificity: Use of cellulose-linked antibodies as immunosorbents.

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