Kinetic studies with skeletal-muscle hexokinase.

The Biochemical journal ·Vol. 100 ·No. 3 ·1966-09-00 ·Pages 739-44

Toews CJ

Abstract

Rat skeletal-muscle hexokinase was partially purified by ammonium sulphate fractionation and gel filtration. The mechanism of the skeletal-muscle hexokinase was studied kinetically by initial-velocity analysis and product inhibition. Glucose 6-phosphate was a non-competitive inhibitor of glucose and ATP. ADP was a non-competitive inhibitor of glucose and a competitive inhibitor of ATP. The data on product inhibition and initial-velocity analysis of skeletal-muscle hexokinase support an ordered sequential mechanism (ordered Bi Bi) where the addition of substrates and release of products is in the order: ATP, glucose, glucose 6-phosphate and ADP.

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Toews C J

Department of Biochemistry, Queen's University, Kingston, Ontario, Canada.

References (15)

15 references, click to expand

The kinetics of enzyme-catalyzed reactions with two or more substrates or products. I. Nomenclature and rate equations.
Biochim Biophys Acta. 1963 Jan 8;67:104-37 PMID: 14021667
Kinetic studies of yeast hexokinase.
J Biol Chem. 1962 Oct;237:3027-32 PMID: 13945795
PRODUCT INHIBITION STUDIES ON YEAST AND LIVER ALCOHOL DEHYDROGENASES.
Biochemistry. 1963 Sep-Oct;2:935-41 PMID: 14087384
EQUILIBRIUM AND NET REACTION RATES IN RELATION TO THE MECHANISM OF YEAST HEXOKINASE.
J Biol Chem. 1964 Nov;239:3645-52 PMID: 14257589
GLUCOKINASE OF RABBIT LIVER.
J Biol Chem. 1965 Mar;240:1014-8 PMID: 14284695
A comparative study of hexokinase from yeast and animal tissues.
J Biol Chem. 1950 Oct;186(2):763-80 PMID: 14794672
Studies on hexokinase. 1. The hexokinase activity of rat-brain extracts.
Biochem J. 1951 Aug;49(3):339-47 PMID: 14858341
Rat skeletal muscle hexokinase. I. Kinetics and reaction mechanism.
J Biol Chem. 1965 Nov;240(11):4133-9 PMID: 5845815
The hexokinase activity of rat muscle extract and the lability of the Mg-ATP complex.
Biochem J. 1953 Jun;54(3):xxii-xxiii PMID: 13058944
The non-competitive inhibition of brain hexokinase by glucose-6-phosphate and related compounds.
J Biol Chem. 1954 Oct;210(2):597-606 PMID: 13211596
The characteristics of hexokinase from Locusta migratoria muscle.
Biochem J. 1957 Oct;67(2):245-50 PMID: 13471540
Lactic dehydrogenase. V. Inhibition by oxamate and by oxalate.
J Biol Chem. 1959 May;234(5):1143-8 PMID: 13654335
Glutamic dehydrogenase. III. The order of substrate addition in the enzymatic reaction.
J Biol Chem. 1959 Nov;234:2891-6 PMID: 13825045
Kinetic studies of the brain hexokinase reaction.
J Biol Chem. 1962 May;237:1661-7 PMID: 13895500
The kinetics of enzyme-catalyzed reactions with two or more substrates or products. II. Inhibition: nomenclature and theory.
Biochim Biophys Acta. 1963 Feb 12;67:173-87 PMID: 14021668

Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1000739

Published

1966-09-00

Pages

739-44

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1265209

External Links

PubMed source DOI: 10.1042/bj1000739 PMC Full Text View journal details

Analysis Services

Kinetic studies with skeletal-muscle hexokinase.

Contact

WeChat Official Account