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PMID: 1668718 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Stereospecific assignment of beta-methylene protons in larger proteins using 3D 15N-separated Hartmann-Hahn and 13C-separated rotating frame Overhauser spectroscopy.

Journal of biomolecular NMR ·Vol. 1 ·No. 1 ·1991-05-00 ·Pages 13-22

Clore GM, Bax A, Gronenborn AM

Abstract

3J alpha beta coupling constants and complementary nuclear Overhauser data on the intraresidue C alpha H-C beta H distances form an essential part of the data needed to obtain stereospecific assignments of beta-methylene protons in proteins. In this paper we show that information regarding the magnitude of the 3J alpha beta coupling constants can be extracted from a semi-quantitative interpretation of relative peak intensities in a 3D 15N-separated 1H-1H Hartmann-Hahn 1H-15N multiple quantum coherence (HOHAHA-HMQC) spectrum. In addition, we demonstrate that reliable information on the intraresidue C alpha H-C beta H distances, free of systematic errors arising from spin diffusion, can be obtained from a 3D 13C-separated 1H-1H rotating frame Overhauser effect 1H-13C multiple quantum coherence (ROESY-HMQC) spectrum. The applicability of these experiments to larger proteins is illustrated with respect to interleukin-1 beta, a protein of 153 residues and 17.4 kDa molecular weight.

MeSH Terms

Amino Acids/chemistry Interleukin-1/chemistry Magnetic Resonance Spectroscopy/methods Molecular Structure Protein Conformation Protons Stereoisomerism

Chemicals

Amino Acids Interleukin-1 Protons

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Clore G M

Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892.

Bax A

Gronenborn A M

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22 references, click to expand

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Article Info

Journal

Journal of biomolecular NMR

Abbr.

J Biomol NMR

ISSN

0925-2738

DOI

10.1007/BF01874566

Published

1991-05-00

Pages

13-22

Language

English

Region

Netherlands

NLM ID

9110829

Subset

External Links

PubMed source DOI: 10.1007/BF01874566 View journal details

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Stereospecific assignment of beta-methylene protons in larger proteins using 3D 15N-separated Hartmann-Hahn and 13C-separated rotating frame Overhauser spectroscopy.

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