Home LiteratureArticle Details
PMID: 16666144 Published · ppublish English Journal Article

Formation of UDP-Xylose and Xyloglucan in Soybean Golgi Membranes.

Plant physiology ·Vol. 87 ·No. 2 ·1988-06-00 ·Pages 341-5

Hayashi T, Koyama T, Matsuda K

Abstract

Soybean (Glycine max) membranes co-equilibrating with Golgi vesicles in linear sucrose gradients contained UDP-glucuronate carboxy-lyase and xyloglucan synthase activities. Digitonin solubilized and increased the activity of the membrane-bound UDP-glucuronate carboxy-lyase. UDP-xylose did not inhibit the transport of UDP-glucuronate into the lumen of Golgi vesicles but repressed the decarboxylation of the translocated UDP-glucuronate. The results suggest that UDP-glucuronate is transported into the vesicles by a specific carrier and decarboxylated to UDP-xylose within the lumen. On incubation of UDP-[(14)C]glucuronate with Golgi membranes in the presence of UDP-glucose, [(14)C]xylose-labeled xyloglucan was formed. Although the K(m) value of UDP-glucuronate for the decarboxylation was 240 micromolar, the affinity of UDP-glucuronate for xyloglucan formation (31 micromolar) was similar to that of UDP-xylose (28 micromolar), suggesting a high turnover of UDP-xylose. The biosynthesis of UDP-xylose from UDP-glucuronate probably occurs in Golgi membranes, where xyloglucan subsequently forms from UDP-xylose and UDP-glucose.

Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hayashi T
Department of Agricultural Chemistry, Tohoku University, Sendai 980, Japan.
Koyama T
Matsuda K
References (21)
21 references, click to expand
  1. Separation and allosteric properties of two forms of UDP-glucuronate carboxy-lyase.
    J Biol Chem. 1977 Nov 25;252(22):8013-7 PMID: 914860
  2. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples.
    Anal Biochem. 1978 Jun 15;87(1):206-10 PMID: 98070
  3. SEPARATION OF SUGAR NUCLEOTIDES, PHOSPHORIC ESTERS AND FREE SUGARS BY PAPER CHROMATOGRAPHY WITH SOLVENTS CONTAINING BORATES OF ORGANIC BASES.
    J Chromatogr. 1965 May;18:342-8 PMID: 14318660
  4. Germination of soybean embryonic axes: nucleotide sugar metabolism and initiation of growth.
    Plant Physiol. 1979 Dec;64(6):975-81 PMID: 16661117
  5. The isolation of uridine diphosphate derivatives of D-glucose, D-galactose, D-xylose, and L-arabinose from mung bean seedlings.
    J Biol Chem. 1956 Dec;223(2):977-83 PMID: 13385245
  6. Fucosylation of xyloglucan: localization of the transferase in dictyosomes of pea stem cells.
    Plant Physiol. 1987 Jul;84(3):753-6 PMID: 16665516
  7. Biosynthesis of the fucose-containing xyloglucan nonasaccharide by pea microsomal membranes.
    Plant Physiol. 1986 Oct;82(2):379-83 PMID: 16665037
  8. Biosynthesis of uridine diphosphate D-xylose. II. Uridine diphosphate D-glucuronate carboxy-lyase of Cryptococcus laurentii.
    Biochemistry. 1966 Jan;5(1):182-9 PMID: 4287217
  9. Biosynthesis of pentosyl lipids by pea membranes.
    Biochem J. 1984 Feb 1;217(3):791-803 PMID: 6712596
  10. Biosynthesis of xyloglucan in suspension-cultured soybean cells. An assay method for xyloglucan xylosyltransferase and attempted synthesis of xyloglucan from UDP-D-xylose.
    J Biochem. 1981 Jan;89(1):325-8 PMID: 7194336
  11. UDP-glucuronate carboxy-lyase in cultured chondrocytes.
    J Biol Chem. 1977 Oct 10;252(19):6707-10 PMID: 197101
  12. Cooperative action of beta-glucan synthetase and UDP-xylose xylosyl transferase of Golgi membranes in the synthesis of xyloglucan-like polysaccharide.
    Biochim Biophys Acta. 1980 May 22;629(3):431-44 PMID: 6448078
  13. Action pattern and specificity of an amylase from Bacillus subtilis.
    Arch Biochem Biophys. 1963 Mar;100:451-67 PMID: 13974504
  14. Biosynthesis of xyloglucan in suspension-cultured soybean cells. Occurrence and some properties of xyloglucan 4-beta-D-glucosyltransferase and 6-alpha-D-xylosyltransferase.
    J Biol Chem. 1981 Nov 10;256(21):11117-22 PMID: 6457048
  15. Uridine diphosphoglucose dehydrogenase of pea seedlings.
    Biochem J. 1957 Aug;66(4):567-72 PMID: 13459898
  16. Decarboxylation of uridine diphosphate-D-glucuronic acid by an enzyme preparation from hen oviduct.
    Biochem Biophys Res Commun. 1965 Dec 21;21(6):543-6 PMID: 5879463
  17. Transport of sugar nucleotides into rat liver Golgi. A new Golgi marker activity.
    J Biol Chem. 1982 Sep 25;257(18):10811-7 PMID: 7050120
  18. The Structure of Plant Cell Walls: I. The Macromolecular Components of the Walls of Suspension-cultured Sycamore Cells with a Detailed Analysis of the Pectic Polysaccharides.
    Plant Physiol. 1973 Jan;51(1):158-73 PMID: 16658279
  19. Mechanism of galactosylation in the Golgi apparatus. A Chinese hamster ovary cell mutant deficient in translocation of UDP-galactose across Golgi vesicle membranes.
    J Biol Chem. 1986 Jan 5;261(1):96-100 PMID: 3510203
  20. The 4-epimerization and decarboxylation of uridine diphosphate D-glucuronic acid by extracts from Phaseolus aureus seedlings.
    J Biol Chem. 1960 Apr;235:910-3 PMID: 13821949
  21. beta-Glucan Synthetases of Plasma Membrane and Golgi Apparatus from Onion Stem.
    Plant Physiol. 1974 Sep;54(3):333-40 PMID: 16658884
Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.87.2.341
Published
1988-06-00
Pages
341-5
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC1054754
External Links
PubMed source DOI: 10.1104/pp.87.2.341 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2