Daptomycin disrupts membrane potential in growing Staphylococcus aureus.

Antimicrobial agents and chemotherapy ·Vol. 35 ·No. 11 ·1991-11-00 ·Pages 2282-7

Alborn WE, Allen NE, Preston DA

Abstract

Daptomycin (LY146032) caused a calcium-dependent dissipation of the membrane potential (delta psi) in Staphylococcus aureus without noticeably affecting the chemical gradient (delta pH) across the membrane. The effect of daptomycin on membrane energization may account for many of the inhibitory effects on macromolecular biosyntheses and membrane function reported for this antibiotic. Our evidence indicates that the bactericidal activity of daptomycin is dependent on an available delta psi.

MeSH Terms

Anti-Bacterial Agents/chemistry,pharmacology Calcium/physiology Daptomycin Flow Cytometry Hydrogen-Ion Concentration Membrane Potentials/drug effects Peptides/chemistry,pharmacology Peptidoglycan/biosynthesis Staphylococcus aureus/drug effects,growth & development,metabolism

Chemicals

Anti-Bacterial Agents Peptides Peptidoglycan Daptomycin Calcium

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Alborn W E

Infectious Disease Research, Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana 46285.

Allen N E

Preston D A

References (15)

15 references, click to expand

Fluorescence indicates a calcium-dependent interaction between the lipopeptide antibiotic LY146032 and phospholipid membranes.
Biochemistry. 1988 Jun 28;27(13):4639-45 PMID: 2844233
The use of flow dialysis for determinations of deltapH and active transport.
Methods Enzymol. 1979;55:680-8 PMID: 37407
In vitro activity and mechanism of action of A21978C1, a novel cyclic lipopeptide antibiotic.
Antimicrob Agents Chemother. 1985 Mar;27(3):357-62 PMID: 3994349
Inhibition of peptidoglycan biosynthesis in gram-positive bacteria by LY146032.
Antimicrob Agents Chemother. 1987 Jul;31(7):1093-9 PMID: 2821889
The effect of cultural conditions on the activity of LY146032 against staphylococci and streptococci.
J Antimicrob Chemother. 1987 Aug;20(2):213-21 PMID: 2822649
In vitro and in vivo activity of LY 146032, a new cyclic lipopeptide antibiotic.
Antimicrob Agents Chemother. 1986 Oct;30(4):532-5 PMID: 3024560
The lipopeptide antibiotic A21978C has a specific interaction with DMPC only in the presence of calcium ions.
Biochim Biophys Acta. 1989 Oct 2;985(1):60-6 PMID: 2790047
Bacterial uptake of aminoglycoside antibiotics.
Microbiol Rev. 1987 Dec;51(4):439-57 PMID: 3325794
The role of acyl chain character and other determinants on the bilayer activity of A21978C an acidic lipopeptide antibiotic.
Biochim Biophys Acta. 1986 Jul 24;859(2):219-26 PMID: 3730378
Lipoteichoic acid as a new target for activity of antibiotics: mode of action of daptomycin (LY146032).
Antimicrob Agents Chemother. 1990 Jun;34(6):1220-6 PMID: 2168145
The use of valinomycin, nigericin and trichlorocarbanilide in control of the protonmotive force in Escherichia coli cells.
Biochem J. 1983 Apr 15;212(1):105-12 PMID: 6307285
Transport of protons across membranes by weak acids.
Physiol Rev. 1980 Jul;60(3):825-63 PMID: 6248908
Quantitative association between electrical potential across the cytoplasmic membrane and early gentamicin uptake and killing in Staphylococcus aureus.
J Bacteriol. 1984 Mar;157(3):863-7 PMID: 6698939
Membrane potential and gentamicin uptake in Staphylococcus aureus.
Proc Natl Acad Sci U S A. 1982 Nov;79(21):6693-7 PMID: 6959147
Mode of action of the peptide antibiotic nisin and influence on the membrane potential of whole cells and on cytoplasmic and artificial membrane vesicles.
Antimicrob Agents Chemother. 1985 May;27(5):841-5 PMID: 4015074

Article Info

Journal

Antimicrobial agents and chemotherapy

Abbr.

Antimicrob Agents Chemother

ISSN

0066-4804

DOI

10.1128/AAC.35.11.2282

Published

1991-11-00

Pages

2282-7

Language

English

Region

United States

NLM ID

0315061

PMCID

PMC245372

Subset

External Links

PubMed source DOI: 10.1128/AAC.35.11.2282 PMC Full Text View journal details

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Daptomycin disrupts membrane potential in growing Staphylococcus aureus.

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