C(6)-[benzene ring]-indole-3-acetic Acid: a new internal standard for quantitative mass spectral analysis of indole-3-acetic Acid in plants.

Plant physiology ·Vol. 80 ·No. 1 ·1986-01-00 ·Pages 14-9

Cohen JD, Baldi BG, Slovin JP

Abstract

Indole-3-acetic acid (IAA) labeled with (13)C in the six carbons of the benzene ring is described for use as an internal standard for quantitative mass spectral analysis of IAA by gas chromatography/selected ion monitoring. [(13)C(6)]IAA was compared to the available deuterium labeled compounds and shown to offer the advantages of nonexchangeability of the isotope label, high isotopic enrichment, and chromatographic properties identical to that of the unlabeled compound. The utility of [(13)C(6)]IAA for measurement of endogenous IAA levels was demonstrated by analysis of IAA in Lemna gibba G-3.

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Cohen J D

United States Department of Agriculture, Agricultural Research Service, Beltsville, Maryland 20705.

Baldi B G

Slovin J P

References (10)

10 references, click to expand

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Article Info

Journal

Plant physiology

Abbr.

Plant Physiol

ISSN

0032-0889

DOI

10.1104/pp.80.1.14

Published

1986-01-00

Pages

14-9

Language

English

Region

United States

NLM ID

0401224

PMCID

PMC1075048

External Links

PubMed source DOI: 10.1104/pp.80.1.14 PMC Full Text View journal details

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C(6)-[benzene ring]-indole-3-acetic Acid: a new internal standard for quantitative mass spectral analysis of indole-3-acetic Acid in plants.

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